The Synthesis and Processing of Epoxide Hyrolase Gene(s) Using Cloned DNA

国际和平研究所建议从事使他能够研究环氧化物水解酶的活动。该酶对外源化合物的环氧化物代谢物起解毒作用。它存在于肝微粒体内，也存在于小鼠细胞的胞浆中。PI希望通过克隆基因来研究不同形式的酶之间的结构差异，以便研究mRNAs的合成和加工。在规划期间，PI建议：1)组织设立实验室。2)制备c DNA探针。3)筛选cDNA4)克隆环氧化物水解酶基因5)研究了环氧化物水解酶的合成工艺。

The PI proposes to engage in activities that will enable him to study epoxide hydrolase. This enzyme functions in detoxifying epoxide metabolites of xenobiotics. It is found in liver microsomes and in a cytosolic fraction in mouse cells. The PI wants to investigate the structural differences among the various forms of the enzyme by cloning the genes in order to study the synthesis and processing of the mRNAs. During the planning period, the PI proposes to: 1) Organize and setup a laboratory. 2) Prepare cDNA probes. 3) Screen cDNA clones 4) Clone epoxide hydrolase genes 5) Study the synthesis and processing of the epoxide hydrolase.