Ca2+ -Induced Rearrangements of Model and Real Biological Membranes

Ca2 诱导的模型和真实生物膜的重排

• 批准号: 8912912
• 负责人: Gerald Feigenson
• 金额: --
• 依托单位: Cornell University
• 依托单位国家: 美国
• 项目类别: Continuing grant
• 财政年份: 1989
• 资助国家: 美国
• 起止时间: 1989-09-01 至 1992-08-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=8912912&HistoricalAwards=false
• 关键词: Ca2+; Induced; Rearrangements; Model; Real

The goal of this project is to detect calcium induced changes in contact regions of biological membranes just prior to fusion. This aim, as well as the methodology employed, is based on physical-chemical studies of calcium binding between phospholipid lamellae that contain phosphatidylserine. Low-light level microscopy will be used to detect a fluorescence signal from the calcium induced rigid phase, simultaneous by with a phase contrast image, in order to identify the cell biological event that accompanies fluorescence. An increase in cytoplasmic calcium concentration can affect exocytosis, cell movement, cell division, metabolism and other processes. This proposal examines one aspect of exocytosis model and real biological membranes in the binding of calcium between bilayer membranes and the resulting lipid and protein rearrangements. The synthesis of fluorescent phospholipids and the development of low-light level microscope systems make possible a search for the small signal from calcium induced membrane contact regions in fusing biological membranes.

该项目的目标是检测钙引起的变化， 在融合之前的生物膜的接触区域。 这一目标以及所采用的方法基于 磷脂与钙结合的物理化学研究 含有磷脂酰丝氨酸磷脂。 微光 显微镜将用于检测来自 钙诱导刚性相，与a相同时 对比度图像，以便识别细胞生物事件 伴随着荧光。 细胞质钙浓度的增加可以影响 胞吐、细胞运动、细胞分裂、代谢等 流程. 这个提议考察了胞吐模型的一个方面 和真实的生物膜之间的钙结合 双层膜和由此产生的脂质和蛋白质 重新安排 荧光磷脂的合成及应用 微光显微镜系统的发展使得 可能的是寻找钙诱导的小信号 融合生物膜的膜接触区域。