Characterization of Rhodospirillum centenum and Other Novel Anoxyphototrophs

百年红螺菌和其他新型无氧光养生物的表征

• 批准号: 8915037
• 负责人: Howard Gest
• 金额: $ 24万
• 依托单位: Indiana University
• 依托单位国家: 美国
• 项目类别: Continuing Grant
• 财政年份: 1990
• 资助国家: 美国
• 起止时间: 1990-02-01 至 1993-07-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=8915037&HistoricalAwards=false
• 关键词: Characterization; Rhodospirillum; centenum; Other; Novel

The thermotolerant nitrogen-fixing bacterium Rhodospirillum centenum, isolated in 1987, is unique among photosynthetic prokaryotes in that it produces desiccation- and heat- resistant cysts. R. centenum is the only Rhodospirillum species known to be amenable to facile genetic and molecular biological analysis. More than 100 mutants with defects in photosynthetic metabolism have been isolated, and these will be characterized biochemically as part of a major objective, namely, mapping the order of the photosynthesis genes of this organism. Determination of the gene order will provide data of importance for future work on "photochemical reaction centers" and for assessing the possible significance of "lateral gene transfer" in the evolution of photosynthetic systems. Control of expression of photosynthesis genes in R. centenum will also be investigated, especially in regard to effects of molecular oxygen. Because the availability of an increased range of unusual phenotypes provides experimental systems of value for study of a number of fundamental problems, searches for previously unknown biotypes of non- oxygenic photosynthetic bacteria will continue using various selective enrichment and other procedures. Anoxygenic photosynthetic bacteria are widely distributed in nature and have been isolated from a great variety of ecological niches. In certain locales they have been identified as primary producers of organic matter and they are prominent catalysts in various geochemical cycles. They have also emerged as outstanding experimental systems for analysis of the basic mechanisms of energy conversion in all types of photosynthetic organisms. It is important to decipher the evolutionary relationships between these organisms. Using both classical genetics and molecular biology, these relationships will be explored with the recently discovered bacterium, Rhodospirillum centenum.

耐热固氮菌Rhodocellum centenum，分离于1987年，是唯一的光合 因为它能产生干燥和热量 抗性包囊。 R. Centenum是唯一一种 已知的物种易于遗传和分子 生物分析 超过100个突变体， 光合代谢已经被分离出来，这些将 作为主要目标的一部分进行生物化学表征， 也就是说，绘制这种植物的光合作用基因的顺序， 有机体 基因顺序的确定将提供数据 对今后“光化学反应”工作的重要性 中心”和评估可能的意义， 光合作用进化中的“横向基因转移” 系统. 光合作用基因表达的调控。 此外，还将对Centenum进行调查，特别是关于 分子氧的影响。 因为， 异常表型范围的增加提供了实验性 价值系统，用于研究一些基本的 问题，搜索以前未知的非生物型， 产氧光合细菌将继续利用各种 选择性浓缩和其他程序。 不产氧光合细菌广泛分布于 大自然，并已从各种各样的隔离 生态位 在某些地方， 被认为是有机物质的主要生产者， 是各种地球化学循环的重要催化剂。 他们 也成为杰出的实验系统， 能量转换的基本机制分析 光合生物的种类。 重要的是要 破译这些物种之间的进化关系 有机体 利用经典遗传学和分子生物学 生物学，这些关系将被探索与 最近发现的细菌百蕊红菌