Identification of the Mouse Sperm Protein Responsible for Sperm-Egg Recognition

Bleil博士实验室的主要兴趣是分析 哺乳动物受精在分子水平，结合 生物化学和分子遗传学技术。研究 这里提出的是旨在确定哪两个识别 小鼠精子蛋白作为ZP3识别蛋白发挥功能： 也就是说，负责小鼠初级结合精子蛋白 精子进入透明区。在初步研究中，一只老鼠 具有ZP3预期特性的精子“56K双联体”蛋白 识别蛋白已根据亲和力鉴定 色谱法和交联研究。这种蛋白质将 进一步的生化特征。它是由 另一些则与精子半乳糖结合蛋白有关 去唾液酸糖蛋白受体作为小鼠精子蛋白发挥功能 它能识别ZP3这里描述的研究旨在 确定这种大鼠蛋白质是否与小鼠精子有关， 56kD蛋白，并且独立地，是否是脱唾液酸糖蛋白 小鼠精子的受体相关蛋白具有预期的特性 ZP3识别蛋白的长期目标 建议的研究是1）识别和表征哺乳动物 精子蛋白（受精蛋白）， 精子和卵子在卵母细胞水平上的相互作用 2）在分子水平上确定精子如何 “受精蛋白”与它们在细胞中的对应物相互作用， 透明带 %%% 这项工作旨在识别精子上的那些分子， 负责识别卵表面的受体， 使受精得以进行。 %%%

The primary interest of Dr. Bleil's laboratory is analysis of mammalian fertilization at the molecular level, combining techniques of biochemistry and molecular genetics. Studies proposed here are designed to determine which of two identified mouse sperm proteins functions as the ZP3 recognition protein: i.e., the sperm protein responsible for primary binding of mouse sperm to the zone pellucida. In preliminary studies, a mouse sperm "56K doublet" protein having properties expected of the ZP3 recognition protein has been identified on the basis of affinity chromatography and crosslinking studies. This protein will be further characterized biochemically. It has been proposed by others that a sperm galactose-binding protein related to asialoglycoprotein receptor functions as the mouse sperm protein which recognizes ZP3. Studies described here are designed to determine whether this rat protein is related to the mouse sperm 56kD protein and, independently, whether an asialoglycoprotein receptor-related protein of mouse sperm has properties expected for the ZP3 recognition protein. The long term objectives of the proposed study are 1) to identify and characterize mammalian sperm proteins (fertilization proteins") which mediate interaction between sperm and egg at the level of the zona pellucida and 2) to determine, at the molecular level, how sperm "fertilization proteins" interact with their counterparts in the zone pellucida. %%% This work proposes to identify those molecules on sperm that are responsible for recognition of receptors on the egg surface that enable fertilization to take place. %%%