Regulation of Cyanogenesis in Plants

植物中氰生成的调控

• 批准号: 8917176
• 负责人: Jonathan Poulton
• 金额: $ 18万
• 依托单位: University of Iowa
• 依托单位国家: 美国
• 项目类别: Continuing Grant
• 财政年份: 1990
• 资助国家: 美国
• 起止时间: 1990-02-01 至 1993-07-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=8917176&HistoricalAwards=false
• 关键词: Regulation; Cyanogenesis; Plants

In black cherry (Prunus serotina) seeds, large-scale catabolism of the cyanogenic glycoside (R)-amygdalin to HCN occurs only upon tissue disruption. Antibodies will be raised against three glycoproteins (amygdalin hydrolase, prunasin hydrolase, and mandelonitrile lyase) catalyzing amygdalin degradation to determine their tissue and subcellular localizations by immunohistochemical techniques. Together with the localization of amygdalin by subcellular fractionation or immunochemical means, this will reveal the mode of compartmentation preventing cyanogenesis in intact seeds. Preliminary to future research on the genetic regulation of cyanogenesis, the phenomenon of cyanogensis will be followed during fruit development from flowering to maturity by measuring the levels of cyanogenic glycosides and of the above catabolic enzymes. The levels of mRNAs encoding these proteins will also be assessed by in vitro translation studies with methionine labeled with a radioisotope of sulfur. Many commercially important crops cause acute and chronic cyanide poisoning in humans and livestock due to the release of hydrogen cyanide (HCN) from cyanogenic glycosides by endogenous enzymes. This research will investigate the metabolism of cyanogenic glycosides in black cheryy. Detailed knowledge of the biosynthesis, sequestration, and degradation of these glycosides will greatly facilitate efforts to reduce the toxicity of such plants and clarify the role of cyanogenesis in plant-animal interactions.

在黑樱桃（Prunus damnina）种子，大规模catenary 氰苷（R）-苦杏仁苷的HCN只发生在 组织破坏 抗体将针对三个 糖蛋白（苦杏仁苷水解酶、prunasin水解酶和 扁桃腈裂解酶）催化苦杏仁苷降解， 确定其组织和亚细胞定位， 免疫组织化学技术。 加上本地化 通过亚细胞分级分离或免疫化学 这就揭示了隔离预防模式 在完整种子中的氰发生。 关于未来研究的初步报告 氰生成的遗传调节， 在果实发育过程中， 开花到成熟通过测量氰的水平 糖苷和上述分解代谢酶。 水平 编码这些蛋白质的mRNA也将通过体外细胞培养评估。 用放射性同位素标记的甲硫氨酸的翻译研究 硫磺。 许多商业上重要的作物引起急性和慢性氰化物 由于氢气的释放而导致人类和牲畜中毒 氰化物（HCN）从生氰苷内源酶。 本研究将探讨产氰菌的代谢 黑樱桃中的糖苷。 的详细知识 这些糖苷的生物合成、螯合和降解 这将极大地促进降低此类药物毒性的努力， 植物和阐明蓝藻在植物-动物 交互.