Development of Methods for Biomolecular NMR, Structure and Design

该奖项为进一步开发使用提供资金 核磁共振（NMR）的研究3 蛋白质和核酸在溶液中的空间结构。 Gorenstein和他的同事开发的方法， 矩阵/完全松弛矩阵程序（或MORASS），已证明 高生产力。 计划的工作应提高准确性， 这些技术的应用。 生物聚合物三维结构的测定 如蛋白质和核酸，以及它与 聚合物的生物学功能，是现代科学的中心问题。 生物学 最成功的方法来研究大型 像蛋白质这样的分子， 方法. 然而，这些方法仅限于研究 在晶体中发现的高度有序的结构;结果可能或可能 不直接适用于（活性）分子的结构 在溶液中。 许多研究人员现在认为核磁共振的使用是最重要的 一种有前途的研究溶液结构的方法， 各种生物分子。 然而，进一步完善 实验技术和分析工具， 光谱数据的解释对于 这一强大工具的应用。

This award provides funds for the further development of the use of Nuclear Magnetic resonance (NMR) for studies of the 3 dimensional structure of proteins and nucleic acids in solution. The approach developed by Gorenstein and his colleagues, the hybrid matrix/complete relaxation matrix program (or MORASS), has proven highly productive. Planned work should increase the accuracy and ease of application of these techniques. Determination of the three dimensional structure of biopolymers such as proteins and nucleic acids, and its relationship to the biological function of the polymers, is a central problem in modern biology. The most successful approach to the study of large molecules like proteins has come with the use of crystallographic methods. These methods, however, are limited to study of the highly ordered structures found in crystals; the results may or may not be directly applicable to the structure of (active) molecules in solution. Many investigators now see the use of NMR as the most promising approach to studies of the solution structure of a variety of biomolecules. However, further refinement of experimental techniques and the analytical tools needed for interpretation of the spectroscopic data is crucial to progress in the application of this powerful tool.