Hyphopodia of Gaeumannomyces graminis

禾顶囊壳是一种土传丝状真菌， 小麦、大麦和水稻的冠部和根部疾病， 这种植物-病原体相互作用提供了一个很好的 真菌粘附宿主组织的模型。 首先，真菌， 对塑料和植物表面的反应， 专门的附着细胞（菌丝足），至少可以是 部分纯化。 其次，G.禾本科不是专性的 寄生虫，可以很容易地培养。 第三，真菌可以 由经典和分子遗传技术操纵。 我们的目标之一是确定菌丝足是否对 真菌致病性的初步工作建议。 基因 用转化质粒DNA破坏将用于产生 缺乏产生菌丝足能力的hypp菌株较多。 经过基因分析后，突变体将被 其特征在于它们的定殖和致病潜力。

Gaeumannomyces graminis, a soil-borne, filamentous fungus, causes crown and root disease of wheat, barley and rice and for several reasons this plant-pathogen interaction provides an excellent model for fungal adhesion to host tissue. First, the fungus, in response to plastic as well as plant surfaces, produces specialized attachment cells (hyphopodia), which can be at least partially purified. Second, G. graminis is not an obligate parasite and can be easily cultured. Third, the fungus can be manipulated by both classical and molecular genetic techniques. One of our goals is to determine if hyphopodia are important for fungal pathogenicity as suggested by preliminary work. Gene disruption with transforming plasmid DNA will be used to generate more hyp strains which lack the ability to produce hyphopodia. After they are genetically analyzed, mutants will be characterized as to their colonizing and pathogenic potential.