Hyphopodia of Gaeumannomyces graminis

禾本科全蚀菌丝足

• 批准号: 9001081
• 负责人: Joan Henson
• 金额: $ 1.5万
• 依托单位: Montana State University
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 1990
• 资助国家: 美国
• 起止时间: 1990-01-15 至 1991-06-30
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=9001081&HistoricalAwards=false
• 关键词: Hyphopodia; Gaeumannomyces; graminis

Gaeumannomyces graminis, a soil-borne, filamentous fungus, causes crown and root disease of wheat, barley and rice and for several reasons this plant-pathogen interaction provides an excellent model for fungal adhesion to host tissue. First, the fungus, in response to plastic as well as plant surfaces, produces specialized attachment cells (hyphopodia), which can be at least partially purified. Second, G. graminis is not an obligate parasite and can be easily cultured. Third, the fungus can be manipulated by both classical and molecular genetic techniques. One of our goals is to determine if hyphopodia are important for fungal pathogenicity as suggested by preliminary work. Gene disruption with transforming plasmid DNA will be used to generate more hyp strains which lack the ability to produce hyphopodia. After they are genetically analyzed, mutants will be characterized as to their colonizing and pathogenic potential.

禾本科Gaeumannomyces graminis是一种土壤传播的丝状真菌，可引起小麦、大麦和水稻的冠和根病，由于多种原因，这种植物-病原体相互作用为真菌粘附宿主组织提供了一个很好的模型。首先，真菌对塑料和植物表面做出反应，产生专门的附着细胞（菌丝），至少可以部分纯化。其次，G. graminis不是专性寄生虫，很容易培养。第三，真菌可以通过经典和分子遗传技术来操纵。我们的目标之一是确定是否菌丝足是重要的真菌致病性建议的初步工作。转化质粒DNA的基因破坏将被用来产生更多缺乏产生菌丝的能力的hyp菌株。在对突变体进行基因分析后，将对其定植和致病潜力进行表征。