Genetic and Cell Biologic Studies of Mechanism of mRNA Exit from the Nucleus

本研究的目的是获得温度敏感的酵母突变株，在该突变株中，从细胞核出口的mRNA在限制性温度下被阻断。在对选定的酵母菌株进行诱变后，将通过一系列筛选程序来选择突变体，这些程序旨在消除那些对蛋白质合成或氨基酸摄取简单的温度敏感的酵母，并鉴定那些继续合成mRNA但不允许它在不允许的温度下离开细胞核的酵母。尽管分子进出细胞核的运输对细胞的生命至关重要，但调节这种运输的机制尚不清楚。此外，尽管信使-RNA退出对所有真核细胞都很重要，但以前还没有人采取遗传方法来解决这个细胞功能的基本问题。因此，成功获得对温度敏感的信使-RNA出口突变体将提供重要的新工具，使这一过程的分子基础得以分析。

The goal of this research is to obtain temperature sensitive yeast mutants in which mRNA exit from the nucleus is blocked at the restrictive temperature. After mutagenesis of selected yeast strains, mutants will be selected through a series of screening procedures designed to eliminate those which are simply temperature sensitive for protein synthesis or amino acid uptake, and to identify those which continue to synthesize mRNA but do not allow it to exit the nucleus at the non-permissive temperature. Although transport of molecules into and out of the cell nucleus is critical for the life of a cell, the mechanisms regulating such transport are not understood. Furthermore, despite the central importance of messenger-RNA exit for all eukaryotic cells, a genetic approach to this fundamental problem of cell function has not previously been taken. Success in obtaining temperature sensitive messenger-RNA exit mutants would therefore provide important new tools that would permit analysis of the molecular basis of this process.