RUI: pH Regulation of Genes in Escherichia coli

RUI：大肠杆菌基因的 pH 调节

• 批准号: 9003751
• 负责人: Joan Slonczewski
• 金额: $ 25.9万
• 依托单位: Kenyon College
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 1990
• 资助国家: 美国
• 起止时间: 1990-09-01 至 1994-02-28
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=9003751&HistoricalAwards=false
• 关键词: RUI; pH; Regulation; Genes; Escherichia

The genetic response to pH change will be investigated in Escherichia coli. External pH is a major environmental parameter for bacteria, and internal pH is significant in terms of bacterial physiology and energy transduction. Two novel pH- regulated loci, isolated as lacZ operon fusions, will be studied: ina inducible by membrane-permeable weak acids and protonophores, and alx inducible by external base. These loci will be cloned and sequenced, and their sequences analyzed for promoters and regulatory sites. The possible regulatory role of acid-sensitive sequences such as H-DNA will be investigated. The pH dependence of ina transcription will be tested in vitro. Regulator genes for ina and alx will be isolated, by screening Tn10 pools for isolates which are either constitutive or uninducible. Mutants exhibiting defective pH homeostasis will be sought.

将在大肠杆菌中研究对 pH 值变化的遗传反应。 外部pH值是细菌的主要环境参数，内部pH值对于细菌生理学和能量传导具有重要意义。 将研究两个新的 pH 调节位点，作为 lacZ 操纵子融合体分离：ina 可通过膜渗透性弱酸和质子载体诱导，alx 可通过外部碱诱导。 这些基因座将被克隆和测序，并分析它们的启动子和调控位点的序列。 将研究 H-DNA 等酸敏感序列的可能调节作用。 将在体外测试ina转录的pH依赖性。 通过筛选 Tn10 池中组成型或不可诱导的分离株，可以分离出 ina 和 alx 的调节基因。 将寻找表现出 pH 稳态缺陷的突变体。