Acquisition of Plant Biotechnolgy Instrumentation

阿拉巴马农工大学是一所历史上的黑人赠地大学，通过系统和渐进的努力，在植物生物学方面发展了全面的研究和培训计划。这一努力最近得到了国家的认可，批准其提供植物和土壤科学博士课程。这是历史上黑人学院和大学（HBCUs）中唯一的此类博士课程。该提案概述了目前正在进行的五个独立研究，涉及六个主要用户和众多研究人员科学家，研究生和本科生。小麦科（小麦、大麦、黑麦、小黑麦）、松科（南松）和松属植物的培养及其细胞遗传学评价。这些工作将利用所需的共聚焦显微镜、倒置显微镜和立体显微镜、低温仪器和生物反应器。利用核酸测序仪、合成仪、激光密度计、凝胶分析仪等加强植物转化研究。调查和操作的抗寒性是一个主题，举例说明正在进行的植物激素和植物生长调节研究计划。可用的液相色谱仪（二极管阵列高效液相色谱和离子色谱）和液体闪烁计数器标记的激素和物质将有助于加强这一研究。

Alabama A & M University, a historically black land grant university, through a systematic and progressive effort has developed a comprehensive research and training program in plant biology. This effort was recently recognized by the State granting it approval to offer a Ph.D. program in Plant and Soil Science. This is the only doctoral program of its kind among historically black colleges and universities (HBCUs). This proposal outlines five individual research that are currently underway involving six primary users and numerous research staff scientists, graduate and undergraduate students. The culture and their cytogenetic evaluation in Triticeae (wheat, barley, rye, triticale), Pinus (Southern pines) and Cossypium species. These efforts will utilize the confocal, inverted and stereomicroscopes, cryogenic apparatus and bioreactor requested. Plant transformation research will strengthen by the nucleic acid sequencer and synthesizer, laser densitometer, and gel analyzers. Investigations and manipulation of cold hardiness is a topic that exemplifies ongoing phytohormone and plant growth regulation studies in the program. Availability of liquid chromatograph (with diode array HPLC and ion chromatography) and liquid scintillation counters for labeled hormones and substances will aid in strengthening this research.