Dissertation Research: A Phylogenetic Analysis of Desmognathine Salamanders Utilizing Ribosomal DNA Restriction Site Maps

论文研究：利用核糖体 DNA 限制性位点图对 Desmognathine 蝾螈进行系统发育分析

• 批准号: 9016652
• 负责人: William Duellman
• 金额: --
• 依托单位: University of Kansas Main Campus
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 1991
• 资助国家: 美国
• 起止时间: 1991-01-15 至 1992-06-30
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=9016652&HistoricalAwards=false
• 关键词: Dissertation; Research; Phylogenetic; Analysis; Desmognathine

Morphological data have not provided a clear picture of the relationships among species of the Desmognathinae a monophyletic subfamily of lungless salamanders, family Plethodontidae. Preliminary data on rDNA restriction site variation and published data from other vertebrate groups indicate that rDNA restriction site mapping can generate valuable new phylogenetic information for desmognathines. Use of the sister group relationship between the Desmognathinae and the Plethodontinae will allow application of the outgroup criterion for ascertaining primitive versus derived restriction sites, and a phylogenetic hypothesis will be formulated based ont he most parsimonious explanation of restriction site evolution. Phylogenetic information from rDNA can provide the basis for the testing of pervious hypotheses of life history evolution in desmognathines and can be used to determine the ancestral life history for desmognathines, an important consideration in testing competing hypotheses on the origin of lunglessness in the Plethodontidae. This study is the first investigation of the entire rDNA array in a monophyletic group of salamanders and will increase knowledge of rDNA evolution in vertebrates.

形态学资料尚未提供无肺蝾螈科单系亚科棘齿螈亚科种间关系的清晰图像。rDNA限制性内切位点变异的初步数据和其他脊椎动物类群的已发表数据表明，rDNA限制性内切位点定位可以为地茅苷提供有价值的新的系统发育信息。利用Desmognathinae和Plethodontinae之间的姐妹类群关系，将允许应用外群标准来确定原始与衍生的限制性位点，并且将根据限制性位点进化的最简洁解释制定系统发育假设。来自rDNA的系统发育信息可以为检验以往关于地鼠苦苷生活史进化的假设提供依据，并可用于确定地鼠苦苷的祖先生活史，这是检验关于齿齿科无肺起源的相互竞争的假设的重要考虑因素。这项研究首次对单系蝾螈的整个rDNA序列进行了调查，并将增加对脊椎动物rDNA进化的认识。