Structure and Function of Proline Permease

脯氨酸通透酶的结构和功能

• 批准号: 9017615
• 负责人: Stanley Maloy
• 金额: $ 15万
• 依托单位: University of Illinois at Urbana-Champaign
• 依托单位国家: 美国
• 项目类别: Continuing Grant
• 财政年份: 1991
• 资助国家: 美国
• 起止时间: 1991-09-01 至 1995-02-28
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=9017615&HistoricalAwards=false
• 关键词: Structure; Function; Proline; Permease

An important aspect of cell growth--how necessary nutrients get into the cell (transport)--is poorly understood in terms of ther actual molecular mechanisms of transport and the proteins responsible for it. The proline transport mutants of Salmonella will be used for studies of the molecular mechanism of sodium- driven transport. By selection for particular classes of mutants, domains of the protease involved in substrate binding and translocation will be identified; with the aid of site directed mutagenesis of the implicated residues it whould be possible to test the predicted roles of certain amino acids and come to understand, with the aid of biochemical and biophysical approaches, the conformational changes necessary for substrate translocation. Elucidation of the structure-function relationships of ion-driven transport proteins should result from such examinations.

细胞生长的一个重要方面--必需的营养物质是如何 进入细胞（运输）--在治疗方面知之甚少。 运输的实际分子机制和蛋白质 沙门氏菌的脯氨酸转运突变体 将用于研究钠的分子机制， 驱动运输。 通过选择特定种类的突变体， 参与底物结合的蛋白酶的结构域， 将确定易位;借助定点 通过对所涉及的残基进行诱变， 测试某些氨基酸的预测作用， 理解，借助生物化学和生物物理方法， 底物移位所必需的构象变化。 离子驱动的结构-功能关系的阐明 运输蛋白质应该从这样的检查中产生。