RUI: The Regulation of a Drosophila Accessory Gland Gene

RUI：果蝇辅助腺基因的调控

• 批准号: 9018062
• 负责人: Kevin Kraus
• 金额: $ 12.1万
• 依托单位: Luther College
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 1991
• 资助国家: 美国
• 起止时间: 1991-03-15 至 1994-08-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=9018062&HistoricalAwards=false
• 关键词: RUI; Regulation; Drosophila; Accessory; Gland

The Drosophila melanogaster accessory gland is a male specific tissue whose cells synthesize and secrete a variety of products which are transferred to females during copulation. These transferred products are involved in several processes in the female. These include increased egg production and oviposition, storage of viable sperm, and lowered receptivity to further mating. Dr. Kraus has obtained a cloned gene which is expressed only in the accessory gland. The RNA encoded by this gene is approximately 400bp in length, making the determination of gene structure and sequence a relatively simple task. The goal of the proposed research is two fold; 1) to define the DNA sequences responsible for the regulation of this gene, and 2) to begin to identify the function of this gene's polypeptide product. Dr. Kraus will first sequence the gene, and then define the sequences responsible for the sex and tissue specific expression via deletion mutagenesis and germline transformation. A polyclonal antibody against the polypeptide will be raised in rabbits. Dr. Kraus will then study this gene's expression during the processes of aging and mating by a combination of Western and Northern analyses. Finally, he will begin to examine the role of the polypeptide in the reproductive process by determining whether or not it is transferred to females.

果蝇的附腺是雄性特有的组织，其细胞在交配过程中合成和分泌各种产物，并转移到雌性体内。这些转移的产物在雌性体内参与了几个过程。这些包括增加产卵量和产卵量，储存可存活的精子，以及降低进一步交配的接受度。克劳斯博士已经获得了一种只在附腺中表达的克隆基因。该基因编码的RNA长约400bp，因此确定基因结构和序列是一项相对简单的任务。这项拟议研究的目标有两个：1)确定负责该基因调控的DNA序列；2)开始鉴定该基因的多肽产物的功能。克劳斯博士将首先对基因进行测序，然后通过缺失突变和生殖系转化来确定负责性别和组织特异性表达的序列。将在兔体内制备针对该多肽的多克隆抗体。然后，克劳斯博士将结合西方和北方的分析，研究该基因在衰老和交配过程中的表达。最后，他将开始研究多肽在生殖过程中的作用，确定它是否转移到雌性体内。