Enzyme and Antibody Catalysts for Polyene Cyclization Reactions

这个奖项是作为约翰博士的启动补助金增量 格里芬的博士后奖学金在化学奖，以支持 他在斯坦福大学的研究成果。博士的推力。 格里芬的研究是分离，纯化， 多烯酶和抗体催化剂的表征 环化反应 将定量纯化的环化酶的酶活性， 确定氨基酸和基因序列。的 环化活性位点将通过亲和标记进行研究 和定向诱变实验。 这将允许 设计和制备具有新的 底物和/或产物特异性。杂双环 免疫原将被合成， 能够启动、引导和终止 二烯环氧化物的环化。一旦抗体催化剂 一旦确定，将对其作用机制进行研究。 这将使我们更好地了解甾醇 生物合成和大分子催化，并可提供 用于甾醇合成的有用催化剂。

This award is made as the starter grant increment of Dr. John Griffin's Postdoctoral Fellowship in Chemistry Award in support of his research at Stanford University. The thrust of Dr. Griffin's research is the isolation, purification and characterization of enzyme and antibody catalysyts for polyene cyclization reactions. Enzymatic activity of purified cyclase will be quantified and the amino acid and gene sequence will be determined. The cyclizing acive site will be studied through affinity labelling and directed mutagenesis experiments. This should permit the design and preparation of engineered enzymes having novel substrate and/or product specificities. Heterobicyclic immunogens will be synthesized which will elicit antibodies capable of initiating, directing and terminating the cyclization of diene epoxides. Once antibody catalysts have been identified, their mechanism of action will be studied. This should lead to a better understanding of sterol biosynthesis and macromolecular catalysis and may provide useful catalysts for the synthesis of sterols.