Enzyme and Antibody Catalysts for Polyene Cyclization Reactions

用于多烯环化反应的酶和抗体催化剂

• 批准号: 9018241
• 负责人: John Griffin
• 金额: $ 3.67万
• 依托单位: Stanford University
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 1990
• 资助国家: 美国
• 起止时间: 1990-09-01 至 1992-02-29
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=9018241&HistoricalAwards=false
• 关键词: Enzyme; Antibody; Catalysts; Polyene; Cyclization

This award is made as the starter grant increment of Dr. John Griffin's Postdoctoral Fellowship in Chemistry Award in support of his research at Stanford University. The thrust of Dr. Griffin's research is the isolation, purification and characterization of enzyme and antibody catalysyts for polyene cyclization reactions. Enzymatic activity of purified cyclase will be quantified and the amino acid and gene sequence will be determined. The cyclizing acive site will be studied through affinity labelling and directed mutagenesis experiments. This should permit the design and preparation of engineered enzymes having novel substrate and/or product specificities. Heterobicyclic immunogens will be synthesized which will elicit antibodies capable of initiating, directing and terminating the cyclization of diene epoxides. Once antibody catalysts have been identified, their mechanism of action will be studied. This should lead to a better understanding of sterol biosynthesis and macromolecular catalysis and may provide useful catalysts for the synthesis of sterols.

该奖项是约翰·格里芬博士化学博士后奖学金的启动资助增量，以支持他在斯坦福大学的研究。Griffin博士的研究重点是多烯环化反应的酶和抗体催化剂的分离、纯化和表征。纯化的环化酶的酶活性将被量化，氨基酸和基因序列将被确定。环化活性位点将通过亲和标记和定向诱变实验进行研究。这将允许设计和制备具有新底物和/或产品特异性的工程酶。杂双环免疫原将被合成，它将引发能够启动、引导和终止环氧二烯环化的抗体。一旦抗体催化剂被确定，它们的作用机制将被研究。这将有助于更好地理解甾醇的生物合成和大分子催化，并可能为甾醇的合成提供有用的催化剂。