Pattern Formation and Gene Regulation in Drosophila

果蝇的模式形成和基因调控

• 批准号: 9018618
• 负责人: Ronald Blackman
• 金额: $ 30万
• 依托单位: University of Illinois at Urbana-Champaign
• 依托单位国家: 美国
• 项目类别: Continuing Grant
• 财政年份: 1991
• 资助国家: 美国
• 起止时间: 1991-03-15 至 1994-08-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=9018618&HistoricalAwards=false
• 关键词: Pattern; Formation; Gene; Regulation; Drosophila

The long term goal of this project is to understand the molecular events underlying tissue growth and development. Working with Drosophila melanogaster, Dr. Blackman is studying the expression of the decapentaplegic (dpp) gene in the group of cells, called imaginal disks, that give rise to much of the adult's epidermis. The dpp gene is a member of the TGF-beta family of secreted growth factors and is required for normal pattern formation in the developing disks. Its expression during disk growth is controlled by a 30 kb cis-regulatory region lying downstream (3') of the transcription unit. Distributed throughout this region are numerous regulatory elements, suggesting that many genes interact with dpp to control its pattern of expression. During the project period, Dr. Blackman proposes to: 1) continue his molecular analysis of the cis-regulatory sequences controlling dpp transcription in the imaginal disks and 2) identify genetically those genes responsible for the trans-acting regulatory factors which interact with these sequences. The products of the TGF-beta family are involved in a broad range of growth regulatory events in mammals, amphibians, and insects. While the vertebrate systems have provided a wealth of information on the cell biology and biochemistry of the factors, Drosophila affords the ideal system for examining the role of these molecules during development.

该项目的长期目标是了解分子 组织生长和发育的潜在事件。使用 黑腹果蝇，布莱克曼博士正在研究 的decapentaplegic（dpp）基因在一组细胞，称为 成虫的大部分表皮都是由成虫的胚盘形成的。 dpp基因是分泌型TGF-β家族的成员， 生长因子和所需的正常模式形成， 显影盘。它在磁盘生长过程中的表达是 由位于下游（3 '）的30 kb顺式调节区控制 转录单位的。分布在这一地区的有 许多调控元件，表明许多基因相互作用， 民进党来控制它的表达方式。在项目 期间，布莱克曼博士建议：1）继续他的分子 DPP顺式调控序列分析 转录在成虫盘和2）确定遗传 那些负责反式作用调节因子的基因 与这些序列相互作用。TGF-β的产物 家族参与了广泛的生长调节事件 在哺乳动物，两栖动物和昆虫中。而脊椎动物系统 提供了大量关于细胞生物学的信息， 生物化学的因素，果蝇提供了理想的系统 来研究这些分子在发育过程中的作用。