Telomeres and Heterochromatin in Drosophila

果蝇的端粒和异染色质

• 批准号: 9018629
• 负责人: Mary Pardue
• 金额: --
• 依托单位: Massachusetts Institute of Technology
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 1991
• 资助国家: 美国
• 起止时间: 1991-09-01 至 1994-02-28
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=9018629&HistoricalAwards=false
• 关键词: Telomeres; Heterochromatin; Drosophila

HeT DNA is a family of complex repeated sequences in the genome of Drosophila melanogaster. The sequences are entirely confined to the telomeres and the pericentric heterochromatin. One subset of the HeT DNA family consists of the HeT-A elements a novel set of retroposon-like elements that have bee found added onto the ends of the four "healed" telomeres that have been studied in Drosophila or they may be able to organize telomere sequences. We propose to test the putative function of HeT DNA sequences by attempting to construct functional Drosophila mini-chromosomes using these sequences. The mini-chromosomes will be transformed into cultured Drosophila cells and assayed for their ability to be maintained and replicated as linear chromosomes. Such chromosomes would allow a direct test of our conclusions about functions of HeT DNA sequences. If we are successful in constructing mini- chromosomes, they will be a useful tool for other studies on Drosophila.***//

HeT DNA 是果蝇基因组中一系列复杂的重复序列。 这些序列完全局限于端粒和中心周异染色质。 HeT DNA 家族的一个子集由 HeT-A 元件组成，这是一组新型的逆转录子样元件，已被发现添加到蜜蜂的四个“愈合”端粒的末端，这些端粒已在果蝇中进行了研究，或者它们可能能够组织端粒序列。 我们建议通过尝试使用这些序列构建功能性果蝇微型染色体来测试 HeT DNA 序列的假定功能。 微型染色体将被转化到培养的果蝇细胞中，并检测其作为线性染色体维持和复制的能力。 这样的染色体可以直接检验我们关于 HeT DNA 序列功能的结论。 如果我们成功构建迷你染色体，它们将成为果蝇其他研究的有用工具。***//