Genetic Transposition

拟议研究项目的长期目标是了解导致转座的序列特异性蛋白质-DNA识别反应的分子基础和调控。这将通过研究IS50/Tn5转座系统来实现。转座元件，如IS50/Tn5，是可以催化多种类型的基因组重排的遗传实体。它们被认为在进化中很重要，并可能导致体细胞突变，如与肿瘤发生相关的突变。将利用遗传学和生物化学方法分析IS50/Tn5转座酶与其靶DNA序列的相互作用、转座酶抑制剂对转座酶活性的调节以及宿主蛋白在转座过程中的作用。

The long-term goals of the proposed research project are to understand the molecular basis of and regulation of sequence specific protein-DNA recognition reactions leading to transposition. This will be pursued by studying the IS50/Tn5 transposition system. Transposable elements, such as IS50/Tn5, are genetic entities which can catalyze numerous types of genome rearrangements. They are thought to have been important in evolution and can lead to somatic cell mutations such as those associated with oncogenesis. Genetic and biochemical approaches will be used to analyze the interaction of IS50/Tn5 transposase with its target DNA sequences, the regulation of transposase activity by its inhibitor and the role of host proteins in the transposition process.