Genetic Transposition

拟议研究项目的长期目标是 了解序列的分子基础和调控 特异性蛋白质-DNA识别反应， 换位 这将通过研究IS 50/Tn 5来实现 换位系统 转座因子，如IS 50/Tn 5， 是可以催化多种类型的基因组的遗传实体 重新安排 他们被认为是重要的， 进化，并可能导致体细胞突变，如 与肿瘤发生有关。 遗传和生物化学方法 分析IS 50/Tn 5转座酶的相互作用 与其靶DNA序列，转座酶的调节 活性及其抑制剂和宿主蛋白质在 换位过程

The long-term goals of the proposed research project are to understand the molecular basis of and regulation of sequence specific protein-DNA recognition reactions leading to transposition. This will be pursued by studying the IS50/Tn5 transposition system. Transposable elements, such as IS50/Tn5, are genetic entities which can catalyze numerous types of genome rearrangements. They are thought to have been important in evolution and can lead to somatic cell mutations such as those associated with oncogenesis. Genetic and biochemical approaches will be used to analyze the interaction of IS50/Tn5 transposase with its target DNA sequences, the regulation of transposase activity by its inhibitor and the role of host proteins in the transposition process.