Instrumentation for Enhancing Undergraduate Genetics, Immunology and Microbiology Laboratories and Student Research

增强本科生遗传学、免疫学和微生物学实验室和学生研究的仪器

• 批准号: 9051961
• 负责人: Frances Makowski
• 金额: $ 3.41万
• 依托单位: University of Portland
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 1990
• 资助国家: 美国
• 起止时间: 1990-04-15 至 1992-09-30
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=9051961&HistoricalAwards=false
• 关键词: Instrumentation; Enhancing; Undergraduate; Genetics; Immunology

The curriculum in biology is being enhanced through the acquisition of an ultracentrifuge and kinetic microplate reader. The equipment is being used in the Genetics, Immunology and Microbiology courses to introduce new exercises which employ modern techniques and principles of critical thinking. The equipment increases the variety of projects students may undertake in the Methods in Research course and in undergraduate student research projects. The ultracentrifuge enables students to purify plasmic DNA for restriction endonuclease double digestion mapping and gene cloning, and to purify prokaryotic and eukaryotic genomic DNA and RNA for Southern and Northern blot analyses with non-radioactive labeled probes. The kinetic microplate reader is used by students for performing the ELISA immunoassay, for cell proliferation studies using the MTT assay, for monoclonal antibody analyses, for the Lowry protein assay and for the screning of sera and cultured supernatants for the presence of antibody following immunological in vivo or in vitro stimulation procedures, respectively. The equipment improves the biology program being offered to students majoring in biology or allied health, many of whom are women. The college will contribute an amount equal to the award.

生物学课程正透过购置 一种超离心和动态酶标仪。该设备 用于遗传学、免疫学和微生物学课程， 采用现代技术和原理的新练习 批判性思维该设备增加了项目的多样性 学生可以参加研究方法课程和 本科生研究项目。超浓缩咖啡机 学生纯化血浆DNA进行限制性内切酶双酶切 酶切作图和基因克隆，并纯化原核和 用于Southern和北方印迹分析的真核基因组DNA和RNA 用非放射性标记的探针。动态酶标仪是 用于学生进行ELISA免疫测定，用于细胞 使用MTT测定法进行单克隆抗体的增殖研究 分析，用于Lowry蛋白测定和用于血清的筛选， 培养的上清液中抗体的存在， 免疫学体内或体外刺激程序。 这些设备改善了提供给学生的生物课程 主修生物学或相关健康，其中许多是妇女。的 学院将提供与奖金相等的金额。