Presidential Young Investigator Award - REU Supplement

首席调查员(PI)打算研究用于药物蛋白生产的表达系统的生物学局限性，最初将重点放在酵母作为模式生物上。在酵母中开发稳定的高拷贝数重组载体和高分泌突变是首要目标。实现这些目标将需要对扩增的遗传元件和异源蛋白质分泌的分子生物学进行基础研究。PI解决这些问题的方法将把传统化学工程的分析工具与分子和细胞生物学的实验技术结合起来。

The Principal Investigator (PI) intends to study the biological limitations of expression systems used in the production of pharmaceutical proteins, initially focusing on yeast as a model organism. The development of stable high copy number recombinant vectors and hyper-secreting mutations in yeast are primary goals. Achieving these aims will require fundamental research into the molecular biology of amplified genetic elements and heterologous protein secretion. The PI's approach to these problems will combine the analytical tools of traditional chemical engineering with the experimental techniques of molecular and cellular biology.