Presidential Young Investigator Award: Protein Separations by Adsorption and Molecular Recognition

总统青年研究员奖：通过吸附和分子识别进行蛋白质分离

• 批准号: 9058271
• 负责人: Richard Willson
• 金额: $ 31.25万
• 依托单位: University of Houston
• 依托单位国家: 美国
• 项目类别: Continuing Grant
• 财政年份: 1990
• 资助国家: 美国
• 起止时间: 1990-07-01 至 1996-06-30
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=9058271&HistoricalAwards=false
• 关键词: Presidential; Young; Investigator; Award; Protein

The area of specialization for this PYI award is in separation methods used for production of recombinant proteins. Particular focus will be on protein adsorption and molecular recognition. The research approach combines engineering with the tools of high-resolution separation methods. The research approach combines engineering with the tools of molecular genetics and structural protein chemistry, with the aim of understanding the thermodynamic driving forces and structural basis of association equilibria. While the work is of a fundamental nature, the understanding gained will aid the development of improved separation media and processes. Some specific interests involve a fundamental study of the structural and thermodynamic basis for antibody affinity and ion exchange adsorption of proteins. By using the tools of directed mutagenesis and thermodynamic analysis to probe fundamental driving forces and structural determinants, one will eventually be able to understand separation processes from the most basic level. This understanding will then be used to develop improved separations media and processes.

此奖项的专业领域是 用于生产重组蛋白的方法。 将特别关注蛋白质吸附和分子 识别. 研究方法将工程与 高分辨率分离方法的工具。 研究 这种方法将工程学与分子生物学的工具相结合， 遗传学和结构蛋白化学，目的是 理解热力学驱动力和结构 关联均衡的基础 虽然工作是一个 基本性质，所获得的理解将有助于 开发改进的分离介质和工艺。 一些 具体利益涉及的基本研究 抗体亲和力结构和热力学基础， 蛋白质的离子交换吸附。 通过使用定向突变和热力学的工具 分析以探索基本驱动力和结构 决定因素，人们最终将能够理解 从最基本的层面进行分离。 这 理解将被用于开发改进的 分离介质和工艺。