RUI: Acquisition of an Oligonucleotide Synthesizer

该仪器将用于合成大小从15到30个碱基对的DNA寡核苷酸。这些寡核苷酸将用于以下目的：1)作为双脱氧测序的特异DNA引物，2)作为聚合酶链式反应(PCR)应用的引物，以及3)作为定点突变技术中的引物。所有这三个过程都需要特定序列的寡核苷酸，这些寡核苷酸将用于这些研究项目：1)Morine MHC重组热点的鉴定，2)细菌荧光素酶的分子鉴定，以及3)小鼠新的逆转录病毒测序器家族的分子鉴定。

This instrument will be used for the synthesis of DNA oligonucleotides ranging in size from 15 to 30 bp. These oligonucleotides will be used for the following purposes: 1) as specific DNA primers for dideoxy sequencing, 2) as primers for PCR (polymerase chain reaction) applications, and 3) as primers used in the technique of site-directed mutagenesis. All three of these procedures require oligonucleotides of specific sequence, which will be used in these research projects: 1) characterization of a morine MHC hotspot of recombination, 2) molecular characterization of bacterial luciferase, and 3) molecular characterization of a new family of retroviral sequencer in the mouse.