Biosynthesis of Branched-chain Amino Acids by Methanogenic Bacteria

产甲烷细菌生物合成支链氨基酸

• 批准号: 9103349
• 负责人: William Whitman
• 金额: $ 21万
• 依托单位: University of Georgia Research Foundation Inc
• 依托单位国家: 美国
• 项目类别: Continuing Grant
• 财政年份: 1991
• 资助国家: 美国
• 起止时间: 1991-08-01 至 1995-01-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=9103349&HistoricalAwards=false
• 关键词: Biosynthesis; Branched; chain; Amino; Acids

This research will be focused on the enzyme acetohydroxy acid synthetase--a key regulatory enzyme involved in synthesis of branched chain amino acids--in the archaebacterium Methanococcus. Purification of the enzyme, determination of its physical and chemical properties, and its cloning and its sequence will be the major foci of the study, which will also then address the homology of this enzyme with that of eubacteria and eukaryotes. A nutritional phenomenon termed pseudoauxotrophy, relating to the amino acids leucine and isoleucine will also be investigated. //

本研究将集中在酶乙酰羟酸 合成酶--参与合成的关键调节酶 支链氨基酸--在古细菌甲烷球菌中。 酶的纯化、理化性质的测定和 化学性质，它的克隆和序列将是 研究的主要焦点，这也将解决同源性 与真细菌和真核生物的酶相比较。 一 营养现象称为假营养缺陷型， 还将研究氨基酸亮氨酸和异亮氨酸。 //