Modulation of Oxidative Cross-linking of Plant Cell Wall Structural Proteins: A Novel Site of Regulation in Development and Stress Resistance

植物细胞壁结构蛋白氧化交联的调节：发育和抗逆性调节的新位点

• 批准号: 9104551
• 负责人: Christopher Lamb
• 金额: $ 21万
• 依托单位: The Salk Institute for Biological Studies
• 依托单位国家: 美国
• 项目类别: Continuing Grant
• 财政年份: 1991
• 资助国家: 美国
• 起止时间: 1991-09-15 至 1995-02-28
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=9104551&HistoricalAwards=false
• 关键词: Modulation; Oxidative; Cross; linking; Plant

Elicitor treatment of bean or soybean cell suspension cultures causes rapid cross-linking of preexisting structural proteins in the plant cell wall, following a burst of oxidative metabolism and generation of hydrogen peroxide at the plant cell surface. Oxidative cross-linking is also under developmental control associated with cellular maturation, eg., during hypocotyl growth, and in tissues subject to mechanical stress, eg., the stem-petiole junction. Stimulus-dependent oxidative cross-linking of pre- existing structural proteins in the plant cell wall is a novel site of cellular regulation, with potentially important functions in the flexible realization of the final functional architecture of cell walls during plant development, and rapid toughening of walls in the initial stages of microbial attack or related stresses. Moreover, the rapid response to defined elicitors, which is initiated within 1-2 minutes and complete within 5-10 minutes, provides the basis for functional dissection of the initial steps in the perception and transduction of elicitor signals. The present proposal seeks to explore the regulation of oxidative cross-linking, underlying signal mechanisms and the impact of cross-linking on cell wall properties.//

菜豆或大豆细胞悬浮培养物的激发子处理 导致预先存在的结构蛋白快速交联， 植物细胞壁，经过一系列的氧化代谢， 在植物细胞表面产生过氧化氢。 氧化交联也受到发育的控制 与细胞成熟有关，例如，在下胚轴生长期间， 以及在受到机械应力的组织中，例如，叶柄 交界处。 刺激依赖性氧化交联的前- 植物细胞壁中现有的结构蛋白是一个新位点 细胞调节，具有潜在的重要功能， 最终功能架构的灵活实现 植物发育过程中的细胞壁，以及细胞壁的快速增韧 在微生物攻击或相关压力的初始阶段。 此外，对确定的激发子的快速反应， 在1-2分钟内启动，在5-10分钟内完成， 为初始步骤的功能解剖提供了基础 在激发子信号的感知和传导中。 的 目前的建议旨在探索氧化的调节， 交联，潜在的信号机制和 细胞壁的交联特性。//