Gibberellin Metabolism and Pea Fruit Growth

这项研究的目的是了解种子是如何控制 果皮（豆荚）在豌豆豌豆伸长，在那里它是 知道种子是giberellins的丰富来源， 对荚果伸长有积极影响。 这项研究旨在证实 果皮生长依赖于赤霉素的生物合成， 现场，以确定是否种子，或应用另一个 激素，生长素，在体内调节果皮赤霉素-19氧化酶 活性，并将酶活性水平与果皮 生长，研究种子和生长素的作用机制 刺激赤霉素生物合成，并鉴定种子 促进果皮生长和赤霉素生物合成的因子 在果皮中。 这些研究的实验方法将 涉及使用放射性标记赤霉素前体， 中间体和代谢产物纯化（HPLC法）和分析（ GC-MS。

The goal of this research is to understand how the seed controls pericarp (pod) elongation in the pea Pisium sativum, where it is known that the seed is a rich source of giberellins which have positive effects on pod elongation. This study aims to confirm that pericarp growth is dependent upon giberellin biosynthesis in situ, to determine whether seeds, or application of another hormone, auxin, regulate in vivo pericarp giberellin-19 oxidase activity and relate the level of enzyme activity to pericarp growth, to investigate the mechanism of action of seed and auxin stimulation to giberellin biosynthesis, and to identify the seed factor which promotes pericarp growth and giberellin biosynthesis in the pericarp. Experimental approaches to these studies will involve use of radiolabelled giberellin precursors and intermediates and metabolite purification by HPLC and analysis by GC-MS.