Role of Bicarbonate in Photosystem II Using D1 and D2 Mutants

使用 D1 和 D2 突变体研究碳酸氢盐在光系统 II 中的作用

• 批准号: 9116838
• 负责人: Govindjee
• 金额: $ 5万
• 依托单位: University of Illinois at Urbana-Champaign
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 1992
• 资助国家: 美国
• 起止时间: 1992-07-15 至 1994-12-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=9116838&HistoricalAwards=false
• 关键词: Role; Bicarbonate; Photosystem; II; Using

The long-term goal is to understand the molecular mechanism of quinone reduction by photosynthetic reaction centers. The focus of the work in this area is to define the unique role played by bound bicarbonate anions in plastoquinone reduction and protonation by photosystem II of plants and cyanobacteria. Use of site- directed and site-selected mutants of cyanobacteria has established the involvement of specific amino acids of two separate photosystem II reaction center proteins, D1 and D2, in the binding and functioning of bicarbonate. Using the powerful combination of molecular biology and advanced biophysical techniques, the experimental work proposed here has two specific goals. Additional site-directed mutants of D1 and D2 in Synechocystis sp. PCC 6803 and D1 mutants in Chlamydomonas reinhardtii will be constructed. These will be used to identify the amino acid ligands and structural requirements for bicarbonate binding and resolve the unique role of bicarbonate in the protonation of plastoquinone (i.e., QB- and/or QB2-) and define the identity and role of amino acids involved in this function.

长期目标是了解 通过光合反应中心的醌还原。 重点 这一领域的工作之一是确定以下方面发挥的独特作用： 质体醌还原和质子化中的结合碳酸氢根阴离子 植物和蓝细菌的光系统II。 网站的使用- 定向和定点突变的蓝藻已经建立 两个独立光系统的特定氨基酸的参与 II反应中心蛋白，D1和D2，在结合和 碳酸氢盐的功能。 使用强大的组合， 分子生物学和先进生物物理技术 这里提出的实验工作有两个具体目标。 额外 集胞藻6803 D1和D2定点突变体 并构建莱茵衣藻D1突变体。 这些将用于鉴定氨基酸配体， 碳酸氢盐结合的结构要求，并解决了 碳酸氢盐在质体醌质子化中的独特作用 (i.e., QB-和/或QB 2-），并定义氨基的身份和作用 酸参与这一功能。