Dissertation Research: Molecular Systematic Studies of the Placement of Carnivora Within Eutherian Mammals

论文研究：食肉目在真兽类哺乳动物中的定位的分子系统研究

• 批准号: 9122906
• 金额: $ 1.2万
• 依托单位: Columbia University
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 1992
• 资助国家: 美国
• 起止时间: 1992-04-01 至 1994-03-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=9122906&HistoricalAwards=false
• 关键词: Dissertation; Research; Molecular; Systematic; Studies

This study proposes to examine the placement of the order Carnivora as well as several other groups, among the eighteen extant orders of eutherian mammals. DNA sequence data will be gathered from four different loci: Cytochrome B, Ubiquitin, Histone H3, and the oncogene K-ras. The use of nucleotide sequence data from several different loci, rather than all the sequence of one large gene, yields comparisons of codon bias, rate of evolution, and retention of phylogenetic signal between genes as well as avoiding the evolutionary, peculiarities of any one locus. Each order will be represented by at least two taxa, which will be chosen to be as diverse as possible based on prior systematic analyses. A parsimony approach combined with down- weighting of saturated sites will be used to analyze the data. Additionally, molecular information will be combined with the pertinent morphological data in a separate analysis. This combination of phylogenetic sampling, use of multiple loci, and total information analysis makes it likely that the so far equivocal information on relationships of many of the mammalian orders will be better understood and that current theories of mammalian molecular evolution will be better tested and expanded.

本研究拟探讨订单的安排 食肉动物以及其他几个群体，在十八个 现存的真兽目哺乳动物。 DNA序列数据将被 从四个不同的位点收集：细胞色素B，泛素， 组蛋白H3和癌基因K-ras。 使用核苷酸 来自几个不同基因座的序列数据，而不是所有的 一个大基因的序列，产生密码子偏好的比较， 进化的速度，以及系统发育信号之间的保留 基因以及避免进化，任何特殊性， 一个locus。 每个目至少有两个分类群， 将根据先前的选择尽可能多样化 系统分析。 一种简约的方法结合了向下- 将使用饱和站点的加权来分析数据。 此外，分子信息将与 相关的形态学数据进行单独分析。 这 系统发育抽样的组合，多个基因座的使用，以及 全面信息分析表明，迄今为止， 关于许多哺乳动物之间关系的模棱两可的信息 将更好地理解和目前的理论， 哺乳动物的分子进化将得到更好的测试和扩展。