Cell & Molecular Biology for Undergraduate Biology Majors

细胞

• 批准号: 9152331
• 负责人: Charlotte Zalewsky
• 金额: $ 0.72万
• 依托单位: Carlow College
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 1991
• 资助国家: 美国
• 起止时间: 1991-07-01 至 1993-12-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=9152331&HistoricalAwards=false
• 关键词: Cell; & Molecular; Biology; Undergraduate

The purpose of this project is to update a program in cell and molecular biology at a college that serves adult learners and a significant number of minorities and women. Students are introduced to basic experiments in molecular biology by incorporating modern DNA technology into an existing cell biology course. A microcentrifuge, electrophoresis chambers and shaking water bath allow students to transform E. coli with p-amp and recombinant DNA, isolate DNA, perform DNA restriction digests and analyze their results using electrophoresis. These experiments provide the student with 'hands-on experience' in DNA science.Instruction in cell biology is enhanced by familiarizing students with tools by which cells and their constituent components can be examined, analyzed and documented. A phase contrast microscope allows students to examine living cells, protozoans and bacteria. Electrophoresis units for protein separation are used by students and replace demonstration laboratories. Darkroom equipment (enlarger and ektamatic processor) give students experience in photomicrography and a visual record of their work with isolated organelles, stained chromosomes and transcriptionally active nucleoli, and cytochemical localization of carbohydrates.

这个项目的目的是更新一所大学的细胞和分子生物学课程，为成人学习者和相当数量的少数族裔和女性提供服务。通过将现代DNA技术融入到现有的细胞生物学课程中，向学生介绍分子生物学的基础实验。通过微型离心机、电泳室和摇水浴，学生可以用p-Amp和重组DNA转化大肠杆菌，分离DNA，进行DNA限制性内切酶，并使用电泳法分析结果。这些实验为学生提供了DNA科学的实践经验。通过让学生熟悉检查、分析和记录细胞及其组成成分的工具，细胞生物学的教学得到了加强。相差显微镜使学生能够检查活细胞、原生动物和细菌。用于蛋白质分离的电泳仪被学生使用，取代了示范实验室。暗室设备(放大镜和全息处理机)为学生提供了显微摄影的经验，并直观地记录了他们对分离的细胞器、染色的染色体和转录活性核仁的工作，以及碳水化合物的细胞化学定位。