Presidential Young Investigator Award/Research in Bioinorganic Chemistry

总统青年研究员奖/生物无机化学研究

• 批准号: 9157411
• 负责人: H. Holden Thorp
• 金额: $ 24.85万
• 依托单位: North Carolina State University
• 依托单位国家: 美国
• 项目类别: Continuing Grant
• 财政年份: 1991
• 资助国家: 美国
• 起止时间: 1991-07-15 至 1993-12-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=9157411&HistoricalAwards=false
• 关键词: Presidential; Young; Investigator; Award; Research

Nucleic acid biophysics and DNA-small molecule interactions will be investigated by constructing monolayer electrodes with pendant DNA intercalators such as ethidium and exposing these surfaces to aqueous solutions of nucleic acid. The specific aims are: 1) to assemble and characterize monolayer electrodes with pendant functionalities known to bind DNA, 2) to study the binding of DNA to these electrodes and the biophysics of the bound nucleic acid, 3) to study the interactions of exogenous metallointercalators with the bound DNA, and 4) to investigate the energetics of DNA cleavage by bound drugs such as bleomycin. In a separate but related project use will be made of the diverse, new technology of polymer-modified electrodes to construct model metalloenzymes. In these systems, the electron relay will be provided by the electrode, and the protein environment will be simulated by the polymer film. %%% Dr. H. Holden Thorp of North Carolina State University is supported as a Presidential Young Investigator by the Inorganic, Bioinorganic, and Organometallic Chemistry Program. Dr. Thorp's project is in the area of bioinorganic chemistry and has the key goals of improving our understanding of the interactions of metal complexes with DNA and modeling the structure and function of redox metalloenzymes. Extensive application of recent advances in the preparation of chemically modified electrodes will be made.

通过构建具有悬挂 DNA 嵌入剂（例如乙锭）的单层电极并将这些表面暴露于核酸水溶液中，可以研究核酸生物物理学和 DNA-小分子相互作用。 具体目标是：1) 组装和表征具有已知结合 DNA 的悬垂功能的单层电极，2) 研究 DNA 与这些电极的结合以及结合核酸的生物物理学，3) 研究外源金属嵌入剂与结合 DNA 的相互作用，4) 研究结合药物（如博莱霉素）切割 DNA 的能量学。 在一个单独但相关的项目中，将利用聚合物修饰电极的多种新技术来构建模型金属酶。 在这些系统中，电子中继将由电极提供，蛋白质环境将由聚合物薄膜模拟。 %%% 北卡罗来纳州立大学的 H. Holden Thorp 博士作为总统青年研究员受到无机、生物无机和有机金属化学项目的支持。 Thorp 博士的项目属于生物无机化学领域，其主要目标是提高我们对金属配合物与 DNA 相互作用的理解以及对氧化还原金属酶的结构和功能进行建模。将广泛应用化学修饰电极制备的最新进展。