Presidential Young Investigator Award/Research in Bioinorganic Chemistry

核酸生物物理与DNA-小分子相互作用 将通过构建单层电极进行研究， 悬垂DNA嵌入剂，如乙锭，并暴露这些 核酸的水溶液。 具体 目的是：1）组装和表征单层电极 与已知结合DNA的侧基官能团，2）研究 DNA与这些电极的结合以及 结合核酸，3）研究外源 金属嵌入剂与结合的DNA，和4）调查 结合的药物如 博莱霉素 在一个单独但相关的项目中， 聚合物修饰电极的多样化的新技术 构建模型金属酶。 在这些系统中， 电子继电器将由电极提供， 蛋白质环境将由聚合物膜模拟。 %%% H博士州立大学北卡罗来纳州的霍尔顿索普是 作为总统青年调查员， 无机，生物无机和有机化学课程。 索普博士的项目是在生物无机化学领域 其主要目标是提高我们对 金属配合物与DNA的相互作用， 氧化还原金属酶结构与功能。广泛 最新进展在化学制备中的应用 将制备改性电极。

Nucleic acid biophysics and DNA-small molecule interactions will be investigated by constructing monolayer electrodes with pendant DNA intercalators such as ethidium and exposing these surfaces to aqueous solutions of nucleic acid. The specific aims are: 1) to assemble and characterize monolayer electrodes with pendant functionalities known to bind DNA, 2) to study the binding of DNA to these electrodes and the biophysics of the bound nucleic acid, 3) to study the interactions of exogenous metallointercalators with the bound DNA, and 4) to investigate the energetics of DNA cleavage by bound drugs such as bleomycin. In a separate but related project use will be made of the diverse, new technology of polymer-modified electrodes to construct model metalloenzymes. In these systems, the electron relay will be provided by the electrode, and the protein environment will be simulated by the polymer film. %%% Dr. H. Holden Thorp of North Carolina State University is supported as a Presidential Young Investigator by the Inorganic, Bioinorganic, and Organometallic Chemistry Program. Dr. Thorp's project is in the area of bioinorganic chemistry and has the key goals of improving our understanding of the interactions of metal complexes with DNA and modeling the structure and function of redox metalloenzymes. Extensive application of recent advances in the preparation of chemically modified electrodes will be made.