IS50 Excision and Transposition

这个项目涉及两个实验室的合作，他们正在研究细菌中遗传因子（转座子）从染色体上的一个位置移动到另一个位置的机制。兰佐夫在圣彼得堡的实验室里，戈雷申博士正在研究切除过程，而列兹尼科夫博士正在研究运动过程。转座子Tn5是一种复合细菌转座因子，它由两个几乎相同的IS50序列组成，包含3个抗生素耐药性决定因子。Tn5和IS50的插入被发现是通过一种被称为精确切除的过程去除的，在这种过程中，被打断的基因的结构被恢复到以前的状态。有证据表明这种切除过程与转位过程之间存在关系；尤其是IS50。分析这些关系可以为我们提供关于蛋白质DNA复合物在刺激重组中的作用的基本知识，并可能有助于阐明其机制。

This project involves a collaborative effort of two laboratories that are studying the mechanism by which genetic elements (transposons) move from one location on the chromosome to another in bacteria. Dr. Goryshin in Dr. S. Lanzov's laboratory in St. Petersburg is studying the excision process while Dr. Reznikoff is studying the movement process. The transposon Tn5 is a composite bacterial transposable element which consists of two nearly identical IS50 sequences bracketing 3 antibiotic resistance determinants. Tn5 and IS50 insertions have been found to be removed by a process called precise excision in which the structure of the interrupted gene is restored to its previous state. There exists evidence which suggests a relationship between this excision process and the transposition process; in particular IS50 excised. %%% Analyzing these relationships may provide us with fundamental knowledge regarding the role of protein DNA complexes in stimulating recombination and may help elucidate the mechanisms.