IS50 Excision and Transposition

IS50 切除和转位

• 批准号: 9206801
• 负责人: William Reznikoff
• 金额: $ 4.36万
• 依托单位: University of Wisconsin-Madison
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 1992
• 资助国家: 美国
• 起止时间: 1992-08-15 至 1995-07-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=9206801&HistoricalAwards=false
• 关键词: IS50; Excision; Transposition

This project involves a collaborative effort of two laboratories that are studying the mechanism by which genetic elements (transposons) move from one location on the chromosome to another in bacteria. Dr. Goryshin in Dr. S. Lanzov's laboratory in St. Petersburg is studying the excision process while Dr. Reznikoff is studying the movement process. The transposon Tn5 is a composite bacterial transposable element which consists of two nearly identical IS50 sequences bracketing 3 antibiotic resistance determinants. Tn5 and IS50 insertions have been found to be removed by a process called precise excision in which the structure of the interrupted gene is restored to its previous state. There exists evidence which suggests a relationship between this excision process and the transposition process; in particular IS50 excised. %%% Analyzing these relationships may provide us with fundamental knowledge regarding the role of protein DNA complexes in stimulating recombination and may help elucidate the mechanisms.

该项目涉及两个实验室的合作努力 他们正在研究遗传因素 （转座子）从染色体上的一个位置移动到另一个位置 在细菌中。 Goryshin博士在Dr. S.兰佐夫的实验室， 彼得堡正在研究切除过程， 正在研究运动过程。 转座子Tn 5是一种 复合细菌转座因子，由两个 包含3种抗生素耐药性的几乎相同的IS 50序列 决定因素 已发现Tn 5和IS 50插入是 通过一种称为精确切除的方法去除， 被中断的基因的一部分恢复到原来的状态。 那里 有证据表明，这种切割与 过程和转位过程;特别是IS 50切除。 %%% 分析这些关系可以为我们提供基本的 关于蛋白质DNA复合物在 刺激重组，并可能有助于阐明机制。