Use of Insect Cell Culture for Production of Proteins and Viral Pesticides

使用昆虫细胞培养物生产蛋白质和病毒农药

• 批准号: 9208905
• 负责人: Robert Granados
• 金额: $ 34.72万
• 依托单位: Boyce Thompson Institute Plant Research
• 依托单位国家: 美国
• 项目类别: Continuing Grant
• 财政年份: 1992
• 资助国家: 美国
• 起止时间: 1992-02-01 至 1995-07-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=9208905&HistoricalAwards=false
• 关键词: Use; Insect; Cell; Culture; Production

The insect cell-baculovirus system has received rapid and wide acceptance as a laboratory or small-scale system for production of proteins from recombinant DNA, and an "AIDS vaccine" produced by insect cell systems is in clinical trials. This system offers advantages of safety, high productivity, generally reliable production of active proteins, and a high degree of post-translational processing. Since the post-translational processing machinery of insect cells is not identical to that of mammalian cells, there may be some limitation on the types of proteins that are best produced in insect cell systems. However, products from insect cells typically elicit a strong immunogenic response, and this system appears particularly well-suited for use in vaccine production and possibly for antibodies. In addition, the virus itself may be genetically-engineered to improve its properties as a safe, specific, and effective insecticide against major crop pests and forest insect pests. One of the Principal Investigators (PIs) has conducted the first tests in this country of a field released genetically-engineered virus. The overall objective of this project is the development of a basis for predictable large-scale production of proteins and insecticides using insect cell lines. For either production of proteins or viral pesticides, more efficient production systems will be required. Because these products may be required in large volumes, and are likely to be cost-sensitive (e.g. animal vaccines and bioinsecticides), the success of the system will be strongly dependent on improved and novel engineering design.

昆虫细胞-杆状病毒系统已获得快速和 作为实验室或小规模系统广泛接受， 从重组DNA生产蛋白质，以及一种“艾滋病”， 由昆虫细胞系统生产的“疫苗”已进入临床 审判 该系统具有安全、高效、节能等优点， 生产力，一般可靠的生产活动 蛋白质，以及高度的翻译后 处理. 因为翻译后加工 昆虫细胞的结构与 哺乳动物细胞，可能存在一些类型上的限制， 最适合在昆虫细胞系统中生产的蛋白质。 然而，来自昆虫细胞的产物通常会引起 强烈的免疫原性反应，这个系统似乎 特别适合用于疫苗生产， 可能是抗体。 此外，病毒本身可能是 经过基因工程改造， 安全、特异、有效的杀虫剂， 农作物害虫和森林害虫。 的一个主要 研究人员（PI）已经进行了第一次测试， 一场国家发布的基因工程病毒。 该项目的总体目标是发展 可预测的大规模生产的基础， 蛋白质和杀虫剂。 为 生产蛋白质或病毒杀虫剂， 将需要有效的生产系统。 因为 可能需要大量的这些产品， 可能对成本敏感（例如动物疫苗和 生物杀虫剂），该系统的成功将是 强烈依赖于改进的和新颖的工程 设计