Behavioral and Neuroendocrine Control of Reproduction in a Sex Reversing Frog

性别逆转青蛙繁殖的行为和神经内分泌控制

• 批准号: 9210275
• 负责人: Mary Mendonca
• 金额: $ 1.74万
• 依托单位: Auburn University
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 1992
• 资助国家: 美国
• 起止时间: 1992-07-15 至 1993-12-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=9210275&HistoricalAwards=false
• 关键词: Behavioral; Neuroendocrine; Control; Reproduction; Sex

This study will: 1) explore the generality of the natural sex- reversing ability of Hyperolius viridiflavus in the field. A chromosomal analysis will attempt to determine the frequency of sex-reversed individuals. 2) establish a breeding colony of these frogs. 3) analyze plasma, gonad and larynx samples of the three categories of animals (females, genetic and sex-reversed males) and correlate circulating sec steroid levels to morphological and behavioral changes. 4) compare volumes of hypothalamic brain nuclei of individuals in the three categories and in females treated with testosterone and flutamide, an anti-androgen. 5) test the effectiveness of i.) group composition, ii.) exogenous testosterone and flutamide and iii.) gonadotropin releasing hormone agonist and antagonist in affecting sex-reversal in females housed in different group situations. 6) record vocalizations of genetic and sex- reversed males, analyze the recording to determine if they differ, and if so, initiate call playback experiments in the field to determine if females distinguish between the two. The data obtained in this preliminary venture will provide a springboard to many future studies ranging from the neuroendocrine to the evolutionary in scope. This unique model system is sure to provide new insights into mechanisms controlling sec at both the proximate and ultimate levels.

本研究将：1）探讨自然性别的共性- 在田间条件下，绿黄金丝桃的逆转能力。 一 染色体分析将试图确定 性反转的人2)建立一个繁殖地 青蛙3)分析三人的血浆、性腺和喉部样本 动物类别（雌性、遗传和性别逆转的雄性）， 将循环sec类固醇水平与形态学和 行为改变4)比较下丘脑脑核团的体积 在这三种类型的个体和接受 睾酮和抗雄激素的氟替卡松5)测试 （一）有效性。组组成，二）外源性睾酮 和fluorescent和iii.）促性腺激素释放激素激动剂和 拮抗剂在影响不同饲养条件下雌性动物的性逆转中的作用 群体情况。6)记录遗传和性别的声音 反转的雄性，分析记录以确定它们是否不同， 如果是，则在现场启动呼叫回放实验， 确定女性是否能区分两者。 在这个初步的冒险中获得的数据将提供一个 许多未来研究的跳板，从神经内分泌 进化的范围。 这种独特的模型系统肯定会 提供了新的见解机制控制证券交易委员会在这两个 最接近的和最后的水平。