Ciliary Neurotrophic Factor Receptors on Neuroblastoma Cells

睫状神经营养因子，CNTF，是一种蛋白质，已知促进 生长的特定种类神经元的存活和分化 以及影响神经胶质细胞的发育。 CNTF作用的基本分子机制不是 知道的 一个简单的，容易获得的系统，人类神经母细胞瘤 肿瘤细胞，将用于确定CNTF受体。 此外，本发明还提供了一种方法， 将采用药理学方法来表征 参与CNTF信号转导的细胞内机制， 影响细胞生理学。 CNTF受体的调节 神经递质和离子通道配体，以及 还将研究佛波酯诱导的细胞分化。 这些研究有望提供关于 关键因素的发展，生存，再生和 神经系统中特定类型细胞的老化。

Ciliary neurotrophic factor, CNTF, is a protein known to promote survival and differentiation of specific classes of neurons grown in culture, as well as to affect the development of glial cells. The basic molecular mechanisms for the effects of CNTF are not known. A simple, easily accessible system, human neuroblastoma tumor cells, will be used to identify CNTF receptors. In addition, a pharmacological approach will be taken to characterize the intracellular mechanisms involved in transducing the CNTF signal to affect cell physiology. The regulation of CNTF receptors by neurotransmitters and ion channel ligands, as well as during phorbol ester induced cell differentiation will also be studied. These studies promise to provide fundamental knowledge about factors crucial to the development, survival, regeneration and aging of specific types of cells in the nervous system.