Structural Study of an RNA-Protein Interaction: The Mom mRNA--Com Protein System

这项建议是为了研究信使核糖核酸和相关蛋白质COM的相互作用。首先，将通过一系列生物物理技术，包括化学和酶敏感性、二维核磁共振和荧光，分别纯化和鉴定mRNA和蛋白质。然后将研究MoM RNA和COM蛋白之间的相互作用。这些相互作用允许mRNA被翻译，并将增加对RNA-蛋白质结构-功能关系的理解。在信使核糖核酸被翻译之前，它会与一种叫做COM的蛋白质结合。这种相互作用改变了RNA的结构，使其能够访问发生翻译起始的位置。RNA和蛋白质将通过各种技术独立研究，然后作为一个复合体进行组合和重新检查。

This proposal is to study the interaction of mRNA and an associated protein, com. The mRNA and the protein will first be purified and characterized individually by a series of biophysical techniques, including chemical and enzymatic susceptibility, 2-dimensional NMR and fluorescence. The interactions between the mom RNA and the com protein will then be investigated. These interactions allow the mRNA to be translated, and will add to understanding of RNA-protein structure-function relationships. %%% Before mRNA is translated, it bind to a protein called com. This interaction changes the structure of the RNA to allow access to the site where initiation of translation occurs. The RNA and protein will be studied independently by a variety of techniques, then combined and re-examined as a complex.