Structural Study of an RNA-Protein Interaction: The Mom mRNA--Com Protein System

RNA-蛋白质相互作用的结构研究：Mom mRNA--Com 蛋白质系统

• 批准号: 9214820
• 负责人: Susan White
• 金额: $ 1.8万
• 依托单位: Bryn Mawr College
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 1992
• 资助国家: 美国
• 起止时间: 1992-08-01 至 1995-01-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=9214820&HistoricalAwards=false
• 关键词: Structural; Study; RNA; Protein; Interaction

This proposal is to study the interaction of mRNA and an associated protein, com. The mRNA and the protein will first be purified and characterized individually by a series of biophysical techniques, including chemical and enzymatic susceptibility, 2-dimensional NMR and fluorescence. The interactions between the mom RNA and the com protein will then be investigated. These interactions allow the mRNA to be translated, and will add to understanding of RNA-protein structure-function relationships. %%% Before mRNA is translated, it bind to a protein called com. This interaction changes the structure of the RNA to allow access to the site where initiation of translation occurs. The RNA and protein will be studied independently by a variety of techniques, then combined and re-examined as a complex.

本研究的目的是研究mRNA与一种蛋白质的相互作用， 相关蛋白，com. mRNA和蛋白质将首先被 通过一系列的纯化和表征， 生物物理技术，包括化学和酶 磁化率、二维NMR和荧光。 的 mom RNA和玉米蛋白质之间的相互作用， 研究了 这些相互作用使mRNA 翻译，并将增加对RNA蛋白质的理解 结构-功能关系。 %%% 在mRNA被翻译之前，它与一种叫做com的蛋白质结合。 这 相互作用改变了RNA的结构， 翻译起始的位点。 rna和 蛋白质将通过各种技术独立研究， 然后结合起来重新审视