Analysis of Microbial Community Structure and Function Alonga Detrital Particle Size Gradient

沿碎屑粒径梯度的微生物群落结构和功能分析

• 批准号: 9216320
• 负责人: Robert Sinsabaugh
• 金额: $ 18万
• 依托单位: Clarkson University
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 1993
• 资助国家: 美国
• 起止时间: 1993-07-01 至 1995-12-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=9216320&HistoricalAwards=false
• 关键词: Analysis; Microbial; Community; Structure; Function

In most streams, community production is sustained by the decomposition of imported plant litter. The early stages of plant litter decomposition have received much study; but little work has focused on the fate of fine organic particles (FPOM), the form in which most particulate detritus moves through stream ecosystems. Utilizing recently developed biochemical techniques, I propose to investigate the structure and activity of FPOM- associated microbial communities in two concurrent field experiments. The first is intended to evaluate a series of basic hypotheses about microbial production, extracellular enzyme activity and community structure over a broad range of conditions. The second is focused specifically on the relationship between FPOM degradation rates and the activity of lignocellulose-degrading enzymes. %%% This work will contribute to our basic understanding of the operation of attached microbial communities, a topic of broad interest. In addition, it may lead to the formulation of models that relate FPOM mass loss, currently difficult to determine in situ, to the activity of lignocellulose-degrading enzymes, a readily quantified measure. Such a model would permit routine monitoring of FPOM degradation rates, an application that could contribute to future studies of ecosystem processes and management.

在大多数溪流中，群落生产是由 进口植物凋落物的分解。 的早期阶段 植物凋落物分解的研究很多，但很少 工作集中于精细有机颗粒（FPOM）的命运， 大多数颗粒碎屑在河流中移动的形式 生态系统 利用最近发展的生物化学技术， 我建议研究FPOM的结构和活性- 两个并行领域中的相关微生物群落 实验 第一个目的是评估一系列基本的 微生物生产假说，胞外酶 活动和社区结构在广泛的范围内， 条件 第二个重点是 FPOM降解速率与活性之间的关系 木质纤维素降解酶。 %%% 这项工作将有助于我们基本了解 附着的微生物群落的操作，一个广泛的话题， 兴趣 此外，它可能会导致模型的制定 这与FPOM质量损失有关，目前难以确定， 原位，木质纤维素降解酶， 易于量化的测量。 这样的模式将允许常规 监测FPOM降解速率，这是一种可以 有助于今后对生态系统过程的研究， 管理