Development of a System for the Isolation of Genes Involved in Cold Adaptation in Antarctic Yeasts

南极酵母冷适应相关基因分离系统的开发

• 批准号: 9220138
• 负责人: Laurie Connell
• 金额: $ 1.8万
• 依托单位: University of Washington
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 1993
• 资助国家: 美国
• 起止时间: 1993-02-15 至 1994-07-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=9220138&HistoricalAwards=false
• 关键词: Development; System; Isolation; Genes; Involved

The extreme environment of Antarctica provides a unique opportunity to study adaptation of organisms to cold as well as dry conditions. This research will focus on an Antarctic yeast (Leucosporidium sp.) as a model system to study cellular function and regulation at the molecular level through the isolation of genes required for cold adaptation. Before genes necessary to examine these processes will be cloned, a transformational system, including a mechanism for the recovery of isolated DNA, will be developed for Leucosporidium. Antibiotic resistance markers on plasmid or cosmid vectors will be utilized to follow the introduction of exogenous DNA into this system and plasmid or cosmid DNA will be re-isolated from the transformed yeast to insure replication fidelity after transformation. This project will lead to the application of the techniques of RNA subtraction and complementation of induced mutations to isolate those genes which are either constitutively regulated by life in the cold or variably regulated for adaptation to a changing cold environment.

南极洲的极端环境为研究生物体对寒冷和干燥条件的适应提供了独特的机会。 这项研究将重点关注南极酵母（Leucosporidium sp.）作为模型系统，通过分离冷适应所需的基因，在分子水平上研究细胞功能和调节。 在克隆检查这些过程所需的基因之前，将为白孢子虫开发一个转化系统，包括回收分离 DNA 的机制。 将利用质粒或粘粒载体上的抗生素抗性标记来跟踪将外源DNA引入该系统，并且将从转化的酵母中重新分离质粒或粘粒DNA以确保转化后的复制保真度。 该项目将应用RNA消减和诱导突变互补技术来分离那些受寒冷生活组成型调节或为适应不断变化的寒冷环境而变化调节的基因。