Structure and Regulation of Pol II Elongation Complexes

Pol II 延伸复合物的结构和调控

• 批准号: 9220186
• 负责人: Robert Landick
• 金额: $ 25.5万
• 依托单位: Washington University
• 依托单位国家: 美国
• 项目类别: Continuing Grant
• 财政年份: 1993
• 资助国家: 美国
• 起止时间: 1993-06-01 至 1996-11-30
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=9220186&HistoricalAwards=false
• 关键词: Structure; Regulation; Pol; II; Elongation

The long-range goal of this project is to define the interactions in the transcription complex that cause arrest and termination by RNA polymerase II in higher eukaryotes. Transcriptional arrest or termination contributes to gene regulation in eukaryotes, but the underlying mechanisms are unknown. To understand these mechanisms, a much better appreciation of the structure of RNA polymerase II transcription elongation complexes, both at arrest sites where elongation normally is efficient, will be required. Methods will be developed to produce defined elongation complexes that contain either pure RNA polymerase II or RNA polymerase II that carries the modifications and auxiliary factors it acquires during initiation at a promoter. Both types of defined elongation complexes will be isolated and their structures probed using a variety of agents that can reveal the configuration of RNA and DNA strands within them. Experiments will be performed to test two models for transcriptional arrest; 1) that altered configuration of the transcript produces arrest and 2) that arrest is caused by bent, or conformationally altered, DNA molecules. %%% This study will produce new insight into how RNA polymerase transcribes the information in genes (DNA) to an RNA product which is required to express genetic information.

这个项目的长期目标是定义 在转录复合体中， 高等真核生物中的RNA聚合酶II。 转录逮捕或 终止有助于真核生物中的基因调控，但 潜在的机制是未知的。 为了理解这些机制， 对RNA聚合酶II结构的更好理解 转录延伸复合物，无论是在逮捕网站， 伸长通常是有效的，将是必需的。 方法将 被开发以产生限定的延伸复合物， 纯的RNA聚合酶II或携带RNA聚合酶II的RNA聚合酶II， 它在启动过程中获得的修改和辅助因素 在一个发起人。 这两种类型的确定的延伸复合物将是 分离并使用各种试剂探测其结构， 可以揭示其中RNA和DNA链的结构。 将进行实验以测试两个模型， 转录停滞; 1）改变的结构， 成绩单产生逮捕和2）逮捕是由弯曲，或 构象改变的DNA分子 %%% 这项研究将对RNA聚合酶如何 将基因（DNA）中的信息转录为RNA产物， 是表达遗传信息所必需的。