a-,b-, and y-Tubuln Genes of Arabidopsis

拟南芥的 a、b 和 y 管基因

• 批准号: 9220363
• 负责人: D.Peter Snustad
• 金额: $ 30万
• 依托单位: University of Minnesota-Twin Cities
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 1993
• 资助国家: 美国
• 起止时间: 1993-08-01 至 1997-01-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=9220363&HistoricalAwards=false
• 关键词: Tubuln; Genes; Arabidopsis

The long-term goal of the proposed research is to understand the roles of microtubule-based components of the cytoskeleton in plant development. Morphogenesis in plants is controlled primarily by processes that establish the planes of cell divisions and the axes of cell elongations, and three microtubule arrays unique to plant cells play central roles in these morphogenetic processes. Furthermore, the absence of centrioles in plant cells makes the potential role of gamma-tubulin in the nucleation of microtubules of particular interest in plants. These investigations focus on the genetic control of microtubule-based processes in the model plant Arabidopsis thaliana, which contains 15 characterized alpha and beta-tubulin genes and at least one gamma-tubulin gene. The specific objectives of the proposed investigations are (1) to use immunofluorescence or immunogold microscopy and gamma-tubulin- specific antibodies to localize gamma tubulin within cells throughout the cell cycle, (2) to characterize spatial and temporal patterns of expression of the gamma tubulin gene(s) and selected beta-tubulin genes, and (3) to investigate the utilization and function of the pollen-localized alpha 1-isoform by means of immunolocalization with our alpha 1-tubulin-specific antibody and by analysis of transgenic plants harboring "antisense RNA" chimeric genes.

该研究的长期目标是了解细胞骨架的微管成分在植物发育中的作用。 植物的形态发生主要由建立细胞分裂平面和细胞伸长轴的过程控制，植物细胞特有的三个微管阵列在这些形态发生过程中发挥着核心作用。 此外，植物细胞中中心粒的缺失使得γ-微管蛋白在微管成核中的潜在作用在植物中受到特别关注。 这些研究的重点是模式植物拟南芥中基于微管的过程的遗传控制，该植物包含 15 个特征性的 α 和 β 微管蛋白基因以及至少一个 γ 微管蛋白基因。 拟议研究的具体目标是（1）使用免疫荧光或免疫金显微镜和γ-微管蛋白特异性抗体在整个细胞周期中定位细胞内的γ微管蛋白，（2）表征γ微管蛋白基因和选定的β-微管蛋白基因表达的空间和时间模式，以及（3）研究 通过使用我们的 α1-微管蛋白特异性抗体进行免疫定位，并通过分析含有“反义 RNA”嵌合基因的转基因植物，确定花粉定位的 α1-亚型。