a-,b-, and y-Tubuln Genes of Arabidopsis

拟议研究的长期目标是了解 植物细胞骨架中微管成分的作用 发展 植物的形态发生主要受 确定细胞分裂平面和轴的过程 以及植物特有的三种微管阵列 细胞在这些形态发生过程中起着核心作用。 此外，植物细胞中中心粒的缺乏使得植物细胞中的蛋白质的含量增加。 微管蛋白在微管成核中的潜在作用 对植物特别感兴趣。 这些调查的重点是 模型中基于微管的过程的遗传控制 植物拟南芥，其中含有15个特征性的α 以及β-微管蛋白基因和至少一种γ-微管蛋白基因。 的 拟议调查的具体目标是（1）使用 免疫荧光或免疫金显微镜和γ-微管蛋白- 在细胞内定位γ微管蛋白的特异性抗体 在整个细胞周期中，（2）表征空间和时间 γ微管蛋白基因的表达模式，并选择 β-微管蛋白基因，和（3）调查利用和 花粉定位α 1-亚型的功能， 用我们的α 1-微管蛋白特异性抗体进行免疫定位， 通过分析含有“反义RNA”嵌合体的转基因植物， 基因.

The long-term goal of the proposed research is to understand the roles of microtubule-based components of the cytoskeleton in plant development. Morphogenesis in plants is controlled primarily by processes that establish the planes of cell divisions and the axes of cell elongations, and three microtubule arrays unique to plant cells play central roles in these morphogenetic processes. Furthermore, the absence of centrioles in plant cells makes the potential role of gamma-tubulin in the nucleation of microtubules of particular interest in plants. These investigations focus on the genetic control of microtubule-based processes in the model plant Arabidopsis thaliana, which contains 15 characterized alpha and beta-tubulin genes and at least one gamma-tubulin gene. The specific objectives of the proposed investigations are (1) to use immunofluorescence or immunogold microscopy and gamma-tubulin- specific antibodies to localize gamma tubulin within cells throughout the cell cycle, (2) to characterize spatial and temporal patterns of expression of the gamma tubulin gene(s) and selected beta-tubulin genes, and (3) to investigate the utilization and function of the pollen-localized alpha 1-isoform by means of immunolocalization with our alpha 1-tubulin-specific antibody and by analysis of transgenic plants harboring "antisense RNA" chimeric genes.