Presidential Young Investigator Award

F1 ATP酶是一种大的膜相关蛋白， 连接两个腺嘌呤之间的平衡反应 核苷酸、三磷酸腺苷和腺苷 二磷酸盐，以及质子穿过 膜的 因此，使用合适的质子电化学 通过跨膜的电位差， 三磷酸腺苷合成。 该系统将 磷固态核磁共振研究 共振 将尝试检测中间体， 反应和测量反应速率。 尝试也将是 用来测定所需的结合金属离子之间的距离 对于反应（锰将被取代， 镁）和核苷酸的磷酸基团。 %%% 这项工作的总体目标是确定结构， 原位酶活性位点的动力学;先前 研究依赖于提取技术， 反映酶上反应物的真实浓度 本身 这项研究的长期目标是扩大核磁共振 实验和测量特定氨基酸的接近度 蛋白质上的残基与结合的腺嘌呤核苷酸结合。

The F1 ATPase is large, membrane-associated protein which links an equilibrium reaction between the two adenine nucleotides, adenosine triphosphate and adenosine diphosphate, and the translocation of protons across the membrane. Thus, with a suitable proton electrochemical potential difference across the membrane it can drive the net synthesis of adenosine triphosphate. This system will be studied by phosphorous solid state nuclear magnetic resonance. Attempts will be made to detect intermediates in the reaction and to measure rates of reaction. Attempts will also be made to determine distances between a bound metal ion required for reactions (manganese will be substituted for magnesium) and the phosphate groups of the nucleotide. %%% The overall goal of this work is to define the structure and kinetics of the active site of the enzyme in situ; previous studies have relied on extraction techniques which may not reflect the true concentrations of reactants on the enzyme itself. Longer term aims of this study are to extend the NMR experiments and measure the proximity of specific amino acid residues on the protein to the bound adenine nucleotides.