Phase Separation of Ternary Protein Water Solutions

本课题是对三元蛋白质水溶液相分离的实验和理论研究。这些溶液将通过静态和动态光散射光谱以及生化色谱和离心方法进行研究。理论研究将使用Carnahan-Starling混合熵和相互作用能的平均场模型来构建系统的吉布斯自由能。这将有可能为解释和预测共存相的相界和相组成提供一条途径。研究结果对生物分子材料的纯化和加工具有一定的指导意义。

This project is an experimental and theoretical investigation of phase separations of ternary aqueous protein solutions. The solutions will be studied by static and dynamic light scattering spectroscopy as well as biochemical chromatographic and centrifugation methods. The theoretical studies will use the Carnahan-Starling mixing entropy and a mean field model for the interaction energies in order to construct a Gibbs free energy for the system. This will potentially provide a route to interpretation and prediction of phase boundaries and compositions of coexisting phases. The results will be of significance for the general area of biomolecular materials purification and processing.