US-Bulgaria Cooperative Research on Oxidative Effects of the Superoxide Radical in Escherichia Coli

欧文博士和美国的保加利亚项目 Fridovich和Stephan Liochev博士 “超氧化物自由基的氧化作用 大肠埃希菌“研究人员提出了一个 新的解释的毒性作用的 超氧自由基，占许多 文献中已有的观察结果， 自己进行实验性的探索。 电流 正统观点认为超氧化物是 过氧化氢和作为铁还原剂 离子。 相反，研究人员认为， 和H2 O2作为直接氧化剂，并考虑Fe（II）， 以其各种连接形式，成为重要的 这些氧化剂的目标。 他们发现 大量的铁-4S含有淀粉酶， 被O2迅速氧化灭活。 的 研究结果应阐明 氧毒性和氧依赖性 紫精、醌、芳基硝基的毒性 化合物和其它氧化还原活性化合物。 生物化学科学的这个项目实现了 科学发展规划目标 保加利亚领先的专家 与美国联合收割机互补 在优势领域集中人才和资源 共同利益和能力。

This US-Bulgaria project between Dr. Irwin Fridovich and Dr. Stephan Liochev is on "Oxidation Effects of the Superoxide Radical in Escherichia Coli." The researchers propose a novel explanation for the toxic effects of the superoxide radical, to account for many observations already in the literature which lend themselves to experimental probing. Current orthodoxy views superoxide as a precursor of hydrogen peroxide and as a reductant for ferric ion. The researchers, in contrast, view O2, O2 and H2O2 as direct oxidants and consider Fe(II), in its various ligated forms, to be an important target for these oxidants. They have found that a number of ı4Fe-4S!-containing dehydrases are rapidly oxidatively inactivated by O2. The results should shed light on the mechanisms of oxygen toxicity and of the oxygen-dependent toxicities of viologens, quinones, aryl nitro compounds and of other redox-active compounds. This project in biochemistry science fulfills the program objective of advancing scientific knowledge by enabling leading experts in Bulgaria and the United States to combine complementary talents and pool resources in areas of strong mutual interest and competence.