Protein Chemistry in the Undergraduate Biochemistry Curriculum

本项目为生物化学/分子生物学和化学学生提供现代蛋白质/肽合成和纯化技术的经验，作为两个学期实验室序列的一部分。采用快速蛋白液相色谱法（FPLC）、多肽合成法和高速梯度离心法。在第一学期，学生通过统一的实验序列来学习所有基本的生化方法，这些实验序列是关于一个单一的、特征明确的蛋白质，细胞色素c。第二学期的课程允许学生使用所学的技术来更独立地探索生化问题。学生帮助设计、合成、分离和表征一种生物活性肽。在整个过程中，学生在他们的项目中使用现代生物化学信息检索和分析方法。本课程的最终目标是使高级学生能够定义问题，评估目前已知的内容，适应已发表的程序，做相关的原创工作，并在专业技术报告中总结他们的结果。

This project gives biochemistry/molecular biology and chemistry students experience in modern protein/peptide synthesis and purification techniques as part of a two-semester laboratory sequence. The methods incorporated into the revision are fast protein liquid chromatography (FPLC), peptide synthesis and high speed gradient centrifugation. In the first semester students learn all of the basic biochemical methods through a unified sequence of experiments on a single, well characterized protein, cytochrome c. The second-semester course allows students to use the techniques learned in a more independent exploration of biochemical problems. Students aid in the design, synthesis, isolation and characterization of a bioactive peptide. Throughout the sequence, students use modern methods of biochemical information retrieval and analysis in their projects. The ultimate goal of this course sequence is to bring advanced students to the point of being able to define a problem, assess what is currently known, adapt a published procedure, do related original work and summarize their results in a professional technical report.