RIMI: "Thiol-Mediated Regulation of Yeast-to-Mycelial Dimorphism in Candida Albicans"

RIMI：“硫醇介导的白色念珠菌酵母菌丝二态性调节”

• 批准号: 9253037
• 负责人: Muthukumaran Gunasekaran
• 金额: $ 39.72万
• 依托单位: Fisk University
• 依托单位国家: 美国
• 项目类别: Continuing Grant
• 财政年份: 1992
• 资助国家: 美国
• 起止时间: 1992-10-01 至 1998-05-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=9253037&HistoricalAwards=false
• 关键词: RIMI; Thiol; Mediated; Regulation; Yeast

Fisk and Wayne State Universities (located in the urban surroundings of Nashville and Detroit, respectively) are "minority institutions" with a firm commitment to improve the entry and retention of minority students in various fields of science and engineering. The overall objectives of the proposed research program are to motivate and train the undergraduate and graduate minority students by (a) teaching the basic principles and applications of different biological and biochemical techniques in life science research (b) providing hands-on-experience with laboratory instrumentation (c) encouraging the students to participate in the proposed research project on "Thiol-mediated regulation of yeast-to-mycelial dimorphism in Candida albicans", providing confidence to independently conduct experiments involving biochemistry and molecular genetics; and (d) providing training in scientific data collection and presentation to their peers in scientific conference or meetings. The specific objectives of the research project are: (1) study the effect of various penetrating and non-penetrating thiol-blockers on the yeast-to-mycelial transition in C. albicans; (2) determine the levels of key enzymes of the v-glutamyl cycle from the yeast and mycelial forms of C. albicans to gain insight into the reason for the rapid decline of intracellular glutathione; (3) The genes encoding the enzyme(s) with altered expression concomitant with the morphological changes will be clones, sequenced and characterized using gene cloning techniques. the gene(s) will be isolated form cDNA and genomic libraries of C. albicans and (4) The cloned gene (s) from C. albicans will be expressed in E. coil, the gene product(s) will be purified and used for molecular and biochemical studies. This project would help to increase the number and improve the levels of training of minorities and women in life science research. The implementation of the Research Initiation in Minority Institution (RIMI) project at Fisk and Wayne State Universities will not only improve the quality of the preparation of the students, which in turn will enhance their chances to enter graduate or professional schools, but also will enable the Biology Department at Fisk to recruit more top quality advanced minority students by providing funds for their support. These students, as research participants, will be effective role models for other predominately black undergraduates at Fisk and Wayne State Universities.

菲斯克和韦恩州立大学（位于城市周边 分别位于纳什维尔和底特律的大学）是“少数民族院校” 坚定地致力于改善进入和保留 科学和工程各个领域的少数民族学生。 的 拟议研究计划的总体目标是激励 培养少数民族本科生和研究生， 教学的基本原理和不同的应用 生命科学研究中的生物和生物化学技术（B） 提供实验室仪器的实际操作经验（c） 鼓励学生参与研究 “硫醇介导的酵母-菌丝体转化调节”项目 白色念珠菌的二型性”，提供信心， 独立进行生物化学实验， 分子遗传学;以及（d）提供科学数据方面的培训 收集并在科学会议上向同行介绍 或会议。 本课题研究的具体目标是：（1）研究 各种渗透性和非渗透性硫醇阻断剂对 C.（2）确定 来自酵母的V-谷氨酰循环的关键酶的水平， 菌丝型C.白色念珠菌，以了解原因 细胞内谷胱甘肽的迅速下降;（3）基因 编码具有伴随所述酶的改变的表达的酶， 形态学变化将被克隆、测序和表征 使用基因克隆技术。 基因将从 C.（4）克隆的基因 (s)梭albicans在E. coil基因 产品将被纯化并用于分子和生物化学 问题研究 该项目将有助于增加数量和改善 少数民族和妇女在生命科学研究方面的培训水平。 少数民族科研创新的实施 菲斯克和韦恩州立大学的机构（RIMI）项目将 不仅提高了学生的准备质量， 这反过来又会增加他们进入研究生或 专业学校，而且还将使生物系在 菲斯克招收更多高素质的先进少数民族学生， 为他们的支持提供资金。 这些学生作为研究 参与者将成为其他主要参与者的有效榜样。 菲斯克和韦恩州立大学的黑人本科生。