U.S.-Argentina: Functional Effect of Mutations Located in the Nucleotide Binding Site of the Plasma Membrane Ca2+ pump

美国-阿根廷：质膜 Ca2 泵核苷酸结合位点突变的功能效应

• 批准号: 9302981
• 负责人: John Penniston
• 金额: $ 1.94万
• 依托单位: Mayo Clinic Rochester
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 1994
• 资助国家: 美国
• 起止时间: 1994-02-15 至 1996-07-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=9302981&HistoricalAwards=false
• 关键词: U.S.; Argentina; Functional; Effect; Mutations

9302981 Penniston This U.S.-Argentina Program award will support the collaboration of John Penniston of the Mayo Foundation and Hugo Adamo of the University of Buenos Aires in Argentina. The project aims to study mutants of the plasma membrane Ca2+ pump. The researchers will make mutants of the plasma membrane Ca2+ pump, express these mutants in COS-1 cells, and study the kinetics of these mutants. They will focus on regions of the pump where alteration of amino acid residues will cause changes in the activity of the pump without totally destroying its activity. Such mutants will provide a residual activity whose study will shed light on the structure-function relationships in the Ca2+ pump and on the mechanisms involved in regulating Ca2+ transport. The U.S. group will benefit from the creation and investigation of Ca2+ pump mutants, and from the Argentine group's expertise in the kinetics of the Ca2+ pump. The Argentine group will gain from the opportunity to make the mutants. ***

9302981 Penniston美国-阿根廷项目奖将支持马约基金会的约翰·彭尼斯顿和阿根廷布宜诺斯艾利斯大学的雨果·阿达莫的合作。 该项目旨在研究质膜Ca 2+泵的突变体。 研究人员将制造质膜Ca 2+泵的突变体，在COS-1细胞中表达这些突变体，并研究这些突变体的动力学。 他们将专注于泵的区域，其中氨基酸残基的改变将导致泵活性的变化，而不会完全破坏其活性。 这些突变体将提供残余活性，其研究将阐明Ca 2+泵的结构-功能关系以及调节Ca 2+转运的机制。 美国小组将受益于钙泵突变体的创造和研究，以及阿根廷小组在钙泵动力学方面的专业知识。 阿根廷队将从制造变种人的机会中获益。 ***