Structural Organization of Proteins on the Oxidizing Side of Photosystem II

这个建议的目的是：(1)鉴定在各种处理后PSII多肽上暴露的和化学敏感的氨基酸残基，以选择性地去除外源蛋白和异质锰簇，(2)鉴定能够被x-连接的蛋白质结构域，(3)表征可能的外域杂三聚体复合体的结构，以及(4)使用振动光谱和圆二色谱来预测每个外源蛋白质的整体二级结构。光合作用过程中的水氧化(放氧)是地球上所有生物的关键反应之一。提出的研究将描述菠菜中与放氧有关的蛋白质之间的相互作用。在有助于理解生物系统中蛋白质-蛋白质和蛋白质-金属离子相互作用的过程中，这些研究可能导致发现形成能够大规模多电子催化的合成络合物的方法。

The aims of this proposal are to: (1) identify the exposed and chemically susceptible amino acid residues on the PSII polypeptides after various treatments to selectively remove extrinsic proteins and heterogeneous Mn clusters, (2) identify protein domains capable of being x-linked, (3) characterize the structure of a possible extrinsic domain hetero-trimer complex, and (4) use vibrational spectroscopy and circular dichroism to predict the overall secondary structure of each extrinsic protein. %%% Water oxidation (oxygen evolution) during photosynthesis is one of the key reactions for all the living creatures on this planet. The studies proposed will characterize the interactions among the proteins involved in oxygen evolution in spinach. In contributing to an understanding of protein-protein and protein-metal ion interactions in living systems these studies may lead to the discovery of approaches to the formation of synthetic complexes capable of multielectron catalysis on large scales.