Structural Organization of Proteins on the Oxidizing Side of Photosystem II

光系统 II 氧化侧蛋白质的结构组织

• 批准号: 9304955
• 负责人: Terry Bricker
• 金额: $ 31.9万
• 依托单位: Louisiana State University
• 依托单位国家: 美国
• 项目类别: Continuing Grant
• 财政年份: 1993
• 资助国家: 美国
• 起止时间: 1993-09-01 至 1997-08-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=9304955&HistoricalAwards=false
• 关键词: Structural; Organization; Proteins; Oxidizing; Side

The aims of this proposal are to: (1) identify the exposed and chemically susceptible amino acid residues on the PSII polypeptides after various treatments to selectively remove extrinsic proteins and heterogeneous Mn clusters, (2) identify protein domains capable of being x-linked, (3) characterize the structure of a possible extrinsic domain hetero-trimer complex, and (4) use vibrational spectroscopy and circular dichroism to predict the overall secondary structure of each extrinsic protein. %%% Water oxidation (oxygen evolution) during photosynthesis is one of the key reactions for all the living creatures on this planet. The studies proposed will characterize the interactions among the proteins involved in oxygen evolution in spinach. In contributing to an understanding of protein-protein and protein-metal ion interactions in living systems these studies may lead to the discovery of approaches to the formation of synthetic complexes capable of multielectron catalysis on large scales.

该提案的目的是：（1）在各种处理后鉴定 PSII 多肽上暴露的和化学敏感的氨基酸残基，以选择性去除外来蛋白质和异质 Mn 簇，（2）鉴定能够 X 连接的蛋白质结构域，（3）表征可能的外在结构域异质三聚体复合物的结构，以及（4）使用振动光谱和循环 二色性来预测每个外在蛋白质的整体二级结构。 %%% 光合作用过程中的水氧化（析氧）是这个星球上所有生物的关键反应之一。 拟议的研究将描述菠菜中参与氧气释放的蛋白质之间的相互作用。 为了有助于理解生命系统中蛋白质-蛋白质和蛋白质-金属离子相互作用，这些研究可能会发现形成能够大规模多电子催化的合成复合物的方法。