Minority Postdoctoral Research Fellowship

9308049拉什这位申请人将研究乙酸乙酯转化为甲烷的甲基转移途径。催化特定甲基转移步骤之一的酶将被提纯和鉴定。将产生对酶的抗体，并将使用免疫金标记来定位细胞中的酶。他们计划克隆和测序该酶的基因(S)，并通过搜索适当的数据库来分析推导出的氨基酸序列，以寻找类似的结构。这项工作将在弗吉尼亚理工学院和州立大学詹姆斯·D·费里博士的实验室进行。***

9308049 Rasche This applicant will study the pathway of methyl transfer in the conversion of acetate to methane. The enzyme catalyzing one of the specific methyl group transfer steps will be purified and characterized. Antibodies will be generated to the enzyme and immunogold labeling will be used to localize the enzyme in the cell. There are plans to clone and sequence the gene(s) for the enzyme, and to analyze the deduced amino acid sequence by searching appropriate databases for similar structures. This work will be conducted in the laboratory of Dr. James D. Ferry at the Virginia Polytechnic Institute and State University. ***