Dissertation Research: Molecular Systematics of Artemisia Sect. Tridentatae

论文研究：蒿属植物分子系统学。

• 批准号: 9311086
• 负责人: Linda Watson
• 金额: $ 0.95万
• 依托单位: University of Oklahoma Norman Campus
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 1993
• 资助国家: 美国
• 起止时间: 1993-08-01 至 1997-01-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=9311086&HistoricalAwards=false
• 关键词: Dissertation; Research; Molecular; Systematics; Artemisia

9311086 Watson Artemesia ( sensu lato ) L. is composed of approximately 400 species distributed throughout the north temperate regions of the world. It is the largest and ecologically most important genus in the Tribe Anthemideae (Asteraceae). The genus has been the subject of numerous comparative biosystematic investigations, but remains poorly understood. Section Tridentatae is native to western North America and plays an important ecological role in the sagebrush communities. Tridentatae is composed of 11 species of xerophytic shrubs, separated from the other North American species by the presence of homogamous flower heads. The rapid expansion and divergence of Tridentatae , coupled with wind pollination has resulted in extensive convergence and parallel evolution. This has made interpretation of phylogenetic relationships difficult. The main objectives of this study are to elucidate interspecific phylogenetic relationships within Tridentatae , and to determine the affinity of Tridentatae to other subgeneric taxa of Artemesia and to the segregate genus Seriphidium . Several molecular techniques will be utilized including phylogenetic analysis of chloroplast DNA and nuclear ribosomal DNA restriction site maps and analysis of sequence data from the ORFk (Open Reading Frame k) region of the cpDNA genome. Previously proposed hypotheses of evolutionary relationships of Tridentatae will be tested using cladistic analyses of molecular data. An examination of concordance between cytoplasmic and nuclear marker based phylogenies of interspecific relationships will be used to test proposed modes of speciation and to examine patterns of character evolution. The inferred phylogenies will be useful in understanding the evolutionary and ecological success of Tridentatae in the cold deserts of western North America. %%% This project will obtain molecular data (both from the chloroplast and nuclear genomes) for 11 species of sagebrush in the Section Tridentatae of the genus Artemesia . These data will be used to decide relationships among these species that have been the subject of some confusion when morphological characters have been used to establish relationships. These woody shrubs are a highly visible and important component of the cold desert ecosystem and understanding species relationships will enhance other research efforts in ecology and biogeography of these plants. ***

9311086 Watson Artemesia (sensu lato) L. 由约 400 个物种组成，分布于世界北温带地区。 它是菊科（Asteraceae）部落中最大且生态上最重要的属。 该属已成为许多比较生物系统研究的主题，但仍然知之甚少。 Tridentatae 原产于北美西部，在山艾树群落中发挥着重要的生态作用。 Tridentatae 由 11 种旱生灌木组成，通过同花头状花序的存在将其与其他北美物种区分开来。 三齿科的快速扩张和分化，加上风授粉，导致了广泛的趋同和平行进化。 这使得系统发育关系的解释变得困难。 本研究的主要目的是阐明三叉齿科内的种间系统发育关系，并确定三叉齿科与蒿属其他亚属类群以及与分离属 Seriphidium 的亲和力。 将利用多种分子技术，包括叶绿体 DNA 和核核糖体 DNA 限制位点图谱的系统发育分析以及 cpDNA 基因组 ORFk（开放阅读框 k）区域的序列数据分析。 先前提出的三叉齿科进化关系的假设将通过分子数据的分支分析进行测试。 对基于种间关系的系统发育的细胞质和核标记之间的一致性的检查将用于测试所提出的物种形成模式并检查性状进化的模式。 推断的系统发育将有助于了解北美洲西部寒冷沙漠中三齿兽科的进化和生态成功。 %%% 该项目将获得蒿属三齿科 11 种山艾树的分子数据（来自叶绿体和核基因组）。 这些数据将用于确定这些物种之间的关系，当使用形态特征来建立关系时，这些物种一直是一些混乱的主题。 这些木本灌木是寒冷沙漠生态系统中高度可见且重要的组成部分，了解物种关系将加强这些植物的生态学和生物地理学方面的其他研究工作。 ***