Equipment and Facilities at a Marine Laboratory

摘要：9311133，Pi-Burris：该项目将帮助海洋生物实验室获得实验室和多用户设备，这些设备将使常驻和访问的科学家以及教育计划受益。将获得一个由两个组件组成的代谢气体分析系统，这将使常规、超高精度测量水样中的二氧化碳和氧气成为可能。该系统的两个组成部分是1)计算机控制的溶解氧(DO)滴定仪，以及2)超高精度的K1溶解无机碳(DIC)或总二氧化碳(TCO2)分析仪。这些仪器将允许精确测定沉积物孔隙水或淡水、滩涂和海洋系统的水柱中的DO(0.1um精度)。利用代谢性气体分析系统进行的研究项目着重关注：海洋和淡水沉积物中有机物质的产生和分解的控制；湖泊、河流、河口和大陆架中微生物食物网和营养结构的变化；北极湖泊、河流和陆缘生态系统中底栖和上层碳和氮的循环；水生系统中溶解有机碳和氮的来源和去向；以及硫酸盐沉积增加导致湖泊发生的生物地球化学变化。代谢气体分析系统将向MBL的所有调查人员提供，并通过广泛分发的MBL宣传册进行宣传。还将购买一个蒸汽高压灭菌器。海洋生物实验室将向来访的科学家和常驻科学家提供这些物品。其次，这些物品还可以用于教育目的。

ABSTRACT: 9311133, PI-Burris: This project will assist the Marine Biological Laboratory in the acquisition of laboratory and multi user equipment that will benefit resident and visi ting scientists and the educational program. A metabolic gas analysis system will be acquired consisting of two components that will enable the routine, ultra high precision measurement carbon dioxide and oxygen in aqueous samples. The two components of the system are 1) a computer controlled dissolved oxygen (DO) titrator, and 2) an ultra high precision K1 dissolved inorganic carbon (DIC) or total CO2 (TCO2) analyzer. These instruments will allow precise determinations of DO (0.1 uM precision) in sediment pore waters or the water column of freshwater, astuarine and marine systems. Research projects benefiting from the acquisition of the metabolic gas analysis system focus on: controls on organic matter production and decomposition in marine and freshwater sediments; microbial food webs and trophic structure of lakes, rivers, estuaries and the continental shelf; benthic and pelagic carbon and nitrogen cycling in arctic lakes and rivers and land margin ecosystems; the source and fate of dissolved organic carbon and nitrogen in aquatic systems; and biogeochemical changes occurring in lakes due to increased sulfate deposition. The metabolic gas analysis system will be available to all investigators at the MBL and advertised through the widely distributed MBL brochures. A steam autoclave will also be acquired. The Marine Biological Laboratory will make these items available to visiting scientists as well as resident scientists. In a secondary manner, the items may also serve educational purposes.