Equipment and Facilities at a Marine Laboratory

摘要：9311133，PI-Burris：该项目将协助海洋生物实验室购置实验室和多用户设备，使常驻和访问科学家以及教育计划受益。将获得代谢气体分析系统，该系统由两个组件组成，可实现常规，超高精度测量含水样品中的二氧化碳和氧气。该系统由两个组成部分组成：1)计算机控制的溶解氧（DO）滴定仪，2)超高精度K1溶解无机碳（DIC）或总二氧化碳（TCO2）分析仪。这些仪器将允许在沉积物孔隙水或淡水、河口和海洋系统的水柱中精确测定DO （0.1 μ m精度）。从获得代谢气体分析系统中受益的研究项目集中在：控制海洋和淡水沉积物中有机物的产生和分解；湖泊、河流、河口和大陆架的微生物食物网和营养结构；北极湖泊、河流和陆地边缘生态系统的底栖和远洋碳氮循环；水生系统中溶解有机碳和氮的来源与去向湖泊中的生物地球化学变化是由于硫酸盐沉积的增加。代谢气体分析系统将提供给MBL的所有研究人员，并通过广泛分发的MBL手册进行宣传。还将获得蒸汽高压灭菌器。海洋生物实验室将向访问科学家和常驻科学家提供这些项目。其次，这些项目也可以用于教育目的。

ABSTRACT: 9311133, PI-Burris: This project will assist the Marine Biological Laboratory in the acquisition of laboratory and multi user equipment that will benefit resident and visi ting scientists and the educational program. A metabolic gas analysis system will be acquired consisting of two components that will enable the routine, ultra high precision measurement carbon dioxide and oxygen in aqueous samples. The two components of the system are 1) a computer controlled dissolved oxygen (DO) titrator, and 2) an ultra high precision K1 dissolved inorganic carbon (DIC) or total CO2 (TCO2) analyzer. These instruments will allow precise determinations of DO (0.1 uM precision) in sediment pore waters or the water column of freshwater, astuarine and marine systems. Research projects benefiting from the acquisition of the metabolic gas analysis system focus on: controls on organic matter production and decomposition in marine and freshwater sediments; microbial food webs and trophic structure of lakes, rivers, estuaries and the continental shelf; benthic and pelagic carbon and nitrogen cycling in arctic lakes and rivers and land margin ecosystems; the source and fate of dissolved organic carbon and nitrogen in aquatic systems; and biogeochemical changes occurring in lakes due to increased sulfate deposition. The metabolic gas analysis system will be available to all investigators at the MBL and advertised through the widely distributed MBL brochures. A steam autoclave will also be acquired. The Marine Biological Laboratory will make these items available to visiting scientists as well as resident scientists. In a secondary manner, the items may also serve educational purposes.