SGER: Mitosis Promoting Factor, A Possible Measure of Instantaneous Growth Rate in Teleost Larvae

渔业生态学的一项主要工作是发展生化工具，以量化瞬时生长速度和吸收幼鱼的营养动态。最近的研究带来了一系列创新技术。由于这些方法在解释上存在问题，目前招募研究的趋势是结合生物化学、组织学和形态计量学技术。因此，我们需要的是一种真正特定的生化指标，可以在任何时间点显示细胞的实际生长速度。细胞周期的生物化学调控的最新进展表明，一种蛋白激酶，有丝分裂促进因子（MPF），作为细胞周期的“主振荡器”。强积金活动的测量表明，强积金作为一种高度特异性的细胞增殖和生长测量方法具有巨大的潜力。为了验证这一想法，DiMichele将开发分析技术，验证非同步细胞群体中有丝分裂的细胞比例，并将理论模型与已知的红鼓鱼（Sciaenops ocellatus）全动物生长速率相匹配。如果该模型得到验证，MPF的生化测量将具有巨大的潜力，可以作为异质群体中瞬时生长和细胞增殖的特定测量。

A major effort in fisheries ecology has been to develop biochemical tools for quantifying instantaneous growth rate and the nutritional dynamics of recruiting fish larvae juveniles. Recent studies have lead to a succession of innovative techniques. Due to problems of interpretation with these methods, current trend in recruitment studies is to use combination of biochemical, histological, and morphometric techniques. Thus, what is needed is a truly specific biochemical indicator of the actual rate of cell growth at any point in time. Recent advances in the biochemical regulation of the cell cycle indicate that a protein kinase, Mitosis Promoting Factor (MPF), acts as the "master oscillator" of the cell cycle. Measurement of MPF activity suggest that MPF has tremendous potential as a highly specific measure of cellular proliferation and growth. To test this idea, DiMichele will develop analytical techniques, verify the proportion of cells committed to mitosis in asynchronous cell populations, and fit the theoretical model to known whole animal growth rates in the red drum, Sciaenops ocellatus. If the model is verified, the biochemical measurement of MPF will have a tremendous potential as a specific measure of instantaneous growth and cellular proliferation in heterogeneous populations.