RUI: Regulation of Transcription Factor Expression in Saccharomyces cerevisiae

RUI：酿酒酵母转录因子表达的调节

• 批准号: 9317062
• 负责人: Barbara Hoopes
• 金额: $ 10.3万
• 依托单位: Colgate University
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 1994
• 资助国家: 美国
• 起止时间: 1994-04-01 至 1997-09-30
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=9317062&HistoricalAwards=false
• 关键词: RUI; Regulation; Transcription; Factor; Expression

9317062 Hoopes Although it is known that the concentration of the general transcription factors are important to gene expression in vivo, very little is known about the expression of the yeast SUA7 gene, which codes for the TFIIB protein of yeast. Using gene fusions and primer extension assays to assess promoter activity, we will examine the regulation of SUA7 under a variety of conditions. This study promises to provide interesting information both on eukaryotic promoter structure and on the effect of the general transcription factors on promoter activity. %%% Cellular regulation often involves the control of RNA synthesis from the DNA template. This type of regulation is essential to maintaining the normal cell state, and understanding gene regulation is important to advances in both medical science and biotechnology. Although we know much about the signals in the DNA that control gene expression, we know less about the regulation of the expression of the molecules (transcription factors) that interact with the DNA. Using yeast as an experimental system, I propose to examine the regulation of a particular transcription factor required for gene expression. This research will contribute to our understanding of mechanisms of gene regulation in higher organisms. ***

小行星9317062 虽然已知一般转录因子的浓度对体内基因表达是重要的，但对编码酵母TFIIB蛋白的酵母SUA7基因的表达知之甚少。 使用基因融合和引物延伸测定来评估启动子活性，我们将研究SUA7在各种条件下的调节。 本研究有望为真核生物启动子结构和一般转录因子对启动子活性的影响提供有趣的信息。 %细胞调节通常涉及控制从DNA模板合成RNA。 这种类型的调节对于维持正常的细胞状态至关重要，理解基因调节对于医学和生物技术的进步都很重要。 虽然我们对DNA中控制基因表达的信号了解很多，但对与DNA相互作用的分子（转录因子）的表达调控知之甚少。 使用酵母作为一个实验系统，我建议检查基因表达所需的一个特定的转录因子的调节。 这一研究将有助于我们了解高等生物的基因调控机制。 ***