Regulation of Glyoxysome Formation During Higher Plant Development

9317526 Harada该项目的长期科学作用是获得对指定乙醛酸酶体功能和结构的过程的机械理解，乙醛酸酶体是将细胞中的关键代谢反应区室化的亚细胞器。 在该提案中，考虑了有关乙醛酸体形成的两个关键问题。 首先，在植物发育过程中，乙醛酸酶体功能的诱导是如何被调节的？ 这个问题将通过解剖调节基因编码的两个乙醛酸体特异性酶，异柠檬酸裂解酶和苹果酸合酶，这是所需的乙醛酸体功能的过程来解决。 该实验室以前的研究确定，转录过程在调节这些酶在乙醛酸酶体中的积累中起主要作用。 因此，这两个基因的表达调控过程的机制的理解将提供洞察的生理信号（S）和途径（S），控制植物发育过程中的乙醛酸酶体功能。 第二个关键问题将集中在乙醛酸体组装。建立细胞器结构和将蛋白质输入过氧化物酶体所需的基因是什么？这些基因在发育过程中是如何调节的？ 将通过选择补充酿酒酵母突变体的植物cDNA克隆来鉴定参与乙醛酸体组装的基因。 从长远来看，这些方法将提供乙醛酸体形成过程的全球概述。 拟议的研究将提供有关建立细胞器结构和功能的过程的重要基础信息。 此外，从这些研究中获得的信息可能直接适用于设计策略，以改善作物的基因工程协议。 ***

9317526 Harada The long range scientific role of this project is to obtain a mechanistic understanding of the processes that specify the function and structure of glyoxysomes, subcellular organelles that compartmentalize critical metabolic reactions in a cell. In this proposal, two key questions about glyoxysome formation are considered. First, how is the induction of glyoxysomal function regulated during plant development? This question will be addressed by dissecting the processes regulating genes encoding two glyoxysome-specific enzymes, isocitrate lyase and malate synthase, that are required for glyoxysomal function. Previous studies from this lab established that transcriptional processes play the primary role in regulating accumulation of these enzymes in glyoxysomes. Therefore, a mechanistic understanding of the processes regulating expression of these two genes will provide insight into the physiological signal(s) and pathway(s) that control glyoxysomal function during plant development. The second key question will focus on glyoxysome assembly. What are the genes that are required to establish the structure of the organelle and to import proteins into peroxisomes, and how are these genes regulated during development? Genes involved in glyoxysome assembly will be identified by selecting plant cDNA clones that complement mutants of Saccharomyces cerevisiae. In the long term, these approaches will provide a global overview of the processes involved in glyoxysome formation. The proposed studies will provide significant basic information about the processes that establish an organelle's structure and function. Furthermore, infomation obtained from these studies may be directly applicable to the design of strategies to improve crop plants using genetic engineering protocals. ***